利拉鲁肽抑制高糖诱导大鼠肾小球系膜细胞株TNF-a和ICAM-1表达

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (12): 1426-1430.

利拉鲁肽抑制高糖诱导大鼠肾小球系膜细胞株TNF-a和ICAM-1表达

孟凡东,李伟

徐州医学院附属医院

收稿日期:2012-02-09 修回日期:2012-03-28 出版日期:2012-12-05 发布日期:2012-11-28
通讯作者: 孟凡东 E-mail:504102802@qq.com

Liraglutide inhibit high glucose-induced TNF-a and ICAM-1 expression in rat glomerular mesangial cells

Received:2012-02-09 Revised:2012-03-28 Online:2012-12-05 Published:2012-11-28

摘要/Abstract

摘要： 目的探讨高糖对大鼠肾小球系膜细胞(RGMC) 中TNF-a和ICAM-1表达影响以及利拉鲁肽的干预作用。方法将培养的RGMC 株分为6 组，分别为：对照组、高糖刺激组、Liraglutide（Li）低、中和高浓度( 10、100和1 000nmol /L) 干预组，以及吡咯烷二硫代氨基甲酸盐( PDTC) 干预组。用ELISA 法测定肾小球系膜细胞TNF-a 以及ICAM-1蛋白表达，用RT-PCR法测定系膜细胞TNF-a 以及ICAM-1基因表达。结果高糖可上调RGMC 中TNF-a、ICAM-1蛋白及基因表达（P均<0.05），利拉鲁肽可抑制高糖诱导下RGMC 中TNF-a、ICAM-1蛋白及基因表达（P均<0.05）。与高糖刺激组比较，PDTC组TNF-a、ICAM-1表达量减少（P<0.05）。结论利拉鲁肽在一定程度上减轻高糖诱导下RGMC 中TNF-a和ICAM-1的表达，可能发挥一定的肾脏保护作用。

Abstract: Objective To explore the effect of Tumor necrosis factor-a(TNF-a) and intercellular adhesion molecule-1 (ICAM-1) in cultured rat glomerular mesangial cells ( RGMC) by high glucose and intervention of Liraglutide. Methods Cultured HBZY21 RGMCs were divided into 6 groups: control cells，high glucose stimulated cells，Liraglutide low、median and high( 10、100、1000nmol/L) concentration incubated cells stimulated with high glucose，PDTC intervention cells. The expressions of TNF-a and ICAM-1 of each group were measured by ELISA and RT-PCR. Results The level of TNF-a and ICAM-1 protein or mRNA was remarkably increased by high glucose，and Liraglutide could prevent this change . Compared with high glucose stimulated cells， the expression of TNF-a and ICAM-1 protein or mRNA was decreased with PDTC intervention cells（P<0.05）.Conclusions Liraglutide might play an important role in preventing diabetes nephropathy by partly relieving the expression of TNF-a and ICAM-1 induced by high glucose.

孟凡东李伟. 利拉鲁肽抑制高糖诱导大鼠肾小球系膜细胞株TNF-a和ICAM-1表达[J]. 基础医学与临床, 2012, 32(12): 1426-1430.