关键词: 骨髓间充质干细胞, 软骨源性形态发生蛋白1, 转化生长因子-β1, 聚乳酸/羟基乙酸共聚物, 软骨分化

Abstract: Objective To explore the repairing methods in vivo and test the clinical applicability of implanatation in vivo of BMSCs in PLGA scafford and induced by cartilage-derived morphogenetic protein 1 (CDMP1) and transforming growth factor-β1 (TGF-β1) to repair defects of cartilage. Method Under the culture of high-density cell suspension and PLGA frame, BMSCs were observed the ability to repair cartilage combined with PLGA before and after the induction of CDMP and (or) TGF-β1. The complex was implanted into animals and the culturing system was analyzed at the gross level, the histological expression to see the ability to repair cartilage defects. Result The culturing system could be induced to express cartilage-specific matrix collagen Ⅱ and GAG. The culturing system could repair cartilage defects effectively after transplanted into animals. Conclusion The BMSCs and PLGA complex induced by CDMP1 and TGF- β1 can repair cartilage defects more effectively than the BMSCs and PLGA complex induced by CDMP1 or TGF-β1 only.