关键词: 短串联重复序列, STR, 细胞库, 标准物质

Abstract: Abstract: Objective To establish and identify a cell line originated from Chinese as reference standards for STR profiling and to establish reference STR-cell bank. Methods The cell morphology of various passages were observed under microscope. The expression of Vimentin and CK was detected immuno- cytochemically. The karyotype were analysed. Contamination of mycoplasma and the species origination of the cell line were checked by PCR. The stability and balance of STR profiling was analysed. Results The cell is fibroblast. The expression of Vimentin is positive, CK is negative. No contamination of mycoplasma was detected. The cell originates from human. All the 15 STR loci were well amplified and demonstrate good balance. STR profiling is completely identical among various passages. Conclusions under 30 passage DNA from these cells can be used as reference standards for STR profiling. These cells were banked as sources of reference DNA standards for STR analysis.

Key words: STR Profiling, STR, Cell banking, reference standards