AMPKα2基因多态性与2型糖尿病及脂联素、抵抗素关系的研究

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (12): 1366-1370.

AMPKα2基因多态性与2型糖尿病及脂联素、抵抗素关系的研究

刘婧星¹,²,任建功²,孙保生¹,¹,罗晖¹,¹,胡雪剑¹,¹,张文博¹,¹

1.
2. 兰州大学第二医院

收稿日期:2010-12-06 修回日期:2011-06-10 出版日期:2011-12-05 发布日期:2011-12-05
通讯作者: 任建功 E-mail:ren-jg@tom.com

Correlation between AMPKα2 subunit gene polymorphism and type 2 diabetes mellitus, adiponectin and resistin

Received:2010-12-06 Revised:2011-06-10 Online:2011-12-05 Published:2011-12-05

摘要/Abstract

摘要： 【摘要】目的：通过研究甘肃地区健康人群和2型糖尿病（T2DM）患者中腺苷酸活化蛋白激酶（AMP-activated protein kinase,AMPK）α2基因单核苷酸多态性（SNPs）位点rs2796516的基因型分布情况，探讨AMPKα2基因多态性与T2DM、胰岛素抵抗及血清脂联素、抵抗素水平的关系。方法：选取甘肃地区T2DM患者188例和非糖尿病正常人群106例，应用聚合酶链反应-限制性片段长度多态性（PCR -RFLP）的方法，检测AMPK-rs 2796516 G/A基因型。应用酶联免疫法（ELISA）测定血清脂联素、抵抗素浓度，并测定血糖、血脂、胰岛素（INS）等指标。结果：（1）T2DM和正常人群中均存在AMPK- rs2796516位点G/A基因突变，二组间基因型构成比及等位基因频率的差异均有统计学意义。（2）T2DM患者中AMPK-rs2796516 G/A基因多态性与胰岛素抵抗、血脂代谢及血清脂联素、抵抗素水平等明显相关，与G/G、G/A组相比, A/A组HOMA- IR明显增高，TG、TCH、LDL增高，血清脂联素水平减低，抵抗素水平增高。结论： AMPK-rs2796516基因多态性与T2DM的发生有明显相关性，推测A等位基因可能是T2DM的易感基因。AMPK-rs2796516基因多态性与其血清脂联素、抵抗素水平有明显相关性，提示脂联素和抵抗素可能通过AMPK参与了胰岛素抵抗，在T2DM的发病过程中起到了一定作用。

关键词: 单磷酸腺苷激活的蛋白激酶, 2型糖尿病, 抵抗素, 脂联素

Abstract: 【Abstract】Objective: To study the genotype distribution of a single nucleotide polymorphism(SNP) rs-2796516(G>A) in 5’-AMP-activated protein kinase(AMPK) alpha-2 subunit gene in gansu area. To investigate the association between AMPKα2 subunit gene polymorphism and type 2 diabetes mellitus(T2DM), insulin resistance(IR) and plasma resistin and adiponectin. Methods: In this study, 188 patientes with T2DM(case group) and 106 healthy persons(control group) from gansu area were enrolled. A SNP rs-2796516(G>A) in AMPKα2 subunit gene was genotyped in all the subjects enrolled using PCR and restriction fragment length polymorphism(RFLP). ELISA technique were employed to determine the plasma adiponectin and resistin concentration. At the same time the fasting blood glucose, insulin and blood lipid were detected. Results: (1)There was polymorphism of AMPK-rs2796516 in both T2DM and normal subjects. There was significant difference of genotype constituent ratio and A allele frequency in two groups. (2) In case group, AMPKα2 subunit gene polymorphism were significantly related to insulin resistance, blood lipid and the concentration of plasma adiponectin and resistin. Compared with GG and GA group, the concentration of serum TG, TCH, LDL, resistin were higher in AA group, the concentration of serum adiponectin was lower in AA group. Conclusion: The polymorphism of AMPK-rs2796516 G/A was revelant with T2DM. It was presumed that A allele maybe the risk gene of T2DM. There was significant association between the polymorphism of AMPK-rs2796516 and the concentration of plasma adiponectin and resistin. Adiponectin and resistin took part in the onset of IR and T2DM through AMPK pathways.

Key words: 5’-AMP-activated protein kinase(AMPK), type 2 diabetes mellitus (T2DM), resistin, adiponectin

刘婧星任建功孙保生罗晖胡雪剑张文博. AMPKα2基因多态性与2型糖尿病及脂联素、抵抗素关系的研究[J]. 基础医学与临床, 2011, 31(12): 1366-1370.

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