基础医学与临床 ›› 2011, Vol. 31 ›› Issue (12): 1358-1362.

• 研究论文 • 上一篇    下一篇

亚临床状态肾上腺皮质肿瘤ACTH受体的表达及临床意义

樊华1,李汉忠1,石冰冰2   

  1. 1. 中国医学科学院 北京协和医学院 北京协和医院泌尿外科
    2. 中国医学科学院 北京协和医学院 北京协和医院
  • 收稿日期:2011-11-02 修回日期:2011-11-07 出版日期:2011-12-05 发布日期:2011-12-05
  • 通讯作者: 李汉忠 E-mail:lihzhpumch@163.com

The expression of ACTH-receptor in sub-clinical adrenocortical neoplasm and its clinical significance

  • Received:2011-11-02 Revised:2011-11-07 Online:2011-12-05 Published:2011-12-05
  • Contact: LI Han-zhong E-mail:lihzhpumch@163.com

摘要: 摘要:目的 对比不同类型肾上腺皮质肿瘤中促肾上腺皮质激素受体(ACTH-R)表达水平的差异,探讨亚临床皮质醇分泌瘤与其他类型肾上腺皮质肿瘤的异同。 方法 应用荧光实时定量PCR检测不同类型肾上腺皮质肿瘤组织中ACTH-R mRNA的表达;比较不同组织中ACTH-R表达的差异,对ACTH-R在肾上腺皮质肿瘤中的表达行相关性分析。 结果 ACTH-R/β-actin mRNA相对比值在正常肾上腺皮质(NC)中为0.961±0.164,醛固酮分泌瘤(APA)中为0.845±0.386,皮质醇分泌瘤(CPA)中为0.636±0.104,亚临床皮质醇分泌瘤(sub-CPA)中为1.242±0.372,肾上腺皮质无功能瘤(NFA)中为1.297±0.228。ACTH-R /β-actin mRNA相对比值在NFA及sub-CPA的表达均显著高于正常肾上腺皮质、APA和CPA(P < 0.01),在CPA中的表达均显著低于其他各组(P <0.05)。 结论 ACTH-R的差异表达可能是亚临床皮质醇分泌瘤内分泌功能低下的决定因素之一。

关键词: 肾上腺皮质肿瘤, ACTH受体, 荧光实时定量多聚酶链式反应

Abstract: Abstract:Objective To analyze different expression levels of ACTH-R in different adrenocortical neoplasms (ANs),and to compare the difference between sub-clinical Cortisol-producing adenoma(sub-CPA) with other ANs. Methods Detect the expression of ACTH-R and internal-reference β-actin mRNA in different ANs tissues through Real time PCR, compare the different expression of ACTH-R mRNA in different tissues, and make correlation analysis on ACTH-R expression in ANs. Results ACTH-R mRNA ratio was 0.961±0.164, 0.845±0.386, 0.636±0.104, 1.242±0.372, and 1.297±0.228 in normal adrenal cortex(NC), aldosterone-producing adenoma (APA), cortisol-producing adenoma (CPA), sub-Clinical Cortisol-producing adenoma(sub-CPA), and Adrenal nonfunctional adenoma(NFA) respectively. ACTH-R mRNA expression was significantly higher in NFA and sub-CPA than in normal adrenal cortex, APA and CPA (P<0.01), was significantly lower in CPA than in other groups (P<0.05). Conclusions Endocrine function of sub-clinical Cortisol-producing adenoma was low, different expression of ACTH-R may be one of factors.

Key words: Adrenocortical neoplasms, ACTH-receptor, real time Polymerase Chain Reaction