Abstract: Objective To observe the effect of the advanced glycation end products (AGEs) on the iNOS expression in cardiac microvascular endothelial cells. Methods Cultured the cardiac microvascular endothelial cells in vitro. After AGEs of the different dose（50～200mg/L）and different times(0～24h) played the role on the cells, we determined the NO generation and iNOS protein expression of all groups. Results NO generation and iNOS protein expression increased with the AGEs -dose increasing and treatment time prolong. Statistical analysis of the results have significantly differences. Conclusin These results demonstrated that AGEs could induce iNOS to produce toxic NO in cardiac microvascular endothelial cells ,then the diabetic cardiomyopathy would occure on clinical.