关键词: 骨形态发生蛋白, 骨肉瘤, 细胞增殖, 细胞凋亡, 细胞迁移, 碱性磷酸酶活性

Abstract: Objective The biological effects of BMP3 on osteosarcoma were investigated by treating human osteosarcoma cell lines MG63, and U2OS with human BMP3 (hBMP3). Methods Osteosarcoma cells in experimental groups were respectively treated with AdBMP-3 and rhBMP3-CM, control groups with AdGFP and rGFP-CM, the blank with neither. The abilities of their proliferation, apoptosis, transmigration and differentiation were respectively detected by trypan blue exclusion test, terminal deoynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and acridine orange-ethidium bromide fluorescent stain (AO/EB), transwell-room and alkaline phosphatase(ALP) activity reagent kit. Results 1) All the indexes detected were not significantly different between two control groups. 2) Compared with control groups, the cell survival rates showed a significant decrease in experimental groups. 3) The apoptosis indexes increased. 4)The number of trans-membrane cell decreased 5) The activity of alkaline phosphatase increased after treating with AdBMP3 and rhBMP-3 for 3 days in MG63, 5 days in U2OS. Conclusions hBMP3 could inhibit osteosarcoma cells and promote bone formation.

Key words: Bone morphogenetic protein, Osteosarcoma, Proliferation, Transmigration, Apoptosis, Alkaline phosphatase