关键词: Par6A, cDNA, L6大鼠骨骼肌细胞, 免疫共沉淀

Abstract: Objective　 The cloning and expression of Par6A. Methods Par6A cDNA was amplified from rat L6 skeletal muscle cells by RT-PCR and the cloning and expression vectors of Par6A were constructed. The expression vector were transfected into 293 cells. Furthermore, the function of Par6A was confirmed by Co-immunoprecipitation. Results Par6A cDNA with approximately 1kb in length was successfully amplified, and the expression vector of pDsRed-Express-N1-Par6A was constructed. The red fluorescene was seen under fluorescent microscope after 293ET cells were transfected for 24h using the pDsRed-Express-N1-Par6A vector. The expressed Par6A protein can interacted with PKCζ.　 Conclusion　 We successfully cloned the Par6A cDNA from rat L6 skeletal muscle cells, which provided a reliable material to study the function of Par6A.

Key words: Par6A, cDNA, rat L6 skeletal muscle cells, Co-immunoprecipitation