基础医学与临床 ›› 2009, Vol. 29 ›› Issue (4): 356-359.

• 研究论文 • 上一篇    下一篇

大鼠肝纤维化组织中PTEN蛋白的定位分布

郝礼森 张晓岚 田晓鹏 安君艳 刘娜   

  1. 河北医科大学第二医院消化内科 河北医科大学第二医院消化内科 河北医科大学第二医院消化内科 河北医科大学第二医院消化内科 河北医科大学第二医院消化内科
  • 收稿日期:2008-05-21 修回日期:2008-10-09 出版日期:2009-04-25 发布日期:2009-04-25
  • 通讯作者: 张晓岚

Distribution and Expression of PTEN Protein of Fibrotic Liver Tissues in Rats

Li-sen HAO, Xiao-lan ZHANG, Xiao-peng TIAN, Jun-yan AN, Na LIU   

  1. Dept. Gastroenterology, the Second Hospital, Hebei Medical University Dept. Gastroenterology, the Second Hospital, Hebei Medical University Dept. Gastroenterology, the Second Hospital, Hebei Medical University Dept. Gastroenterology, the Second Hospital, Hebei Medical University Dept. Gastroenterology, the Second Hospital, Hebei Medical University
  • Received:2008-05-21 Revised:2008-10-09 Online:2009-04-25 Published:2009-04-25
  • Contact: Xiao-lan ZHANG,

摘要: 目的 探讨大鼠纤维化肝组织中PTEN蛋白的定位分布及其在肝纤维化病理过程中的作用。方法 结扎胆总管建立大鼠肝纤维化模型;用免疫组织化学染色测定大鼠肝组织中PTEN及α-SMA 蛋白的分布及表达;免疫荧光双标记共聚焦激光扫描显微术测定大鼠肝组织中活化肝星状细胞(HSC)的PTEN蛋白分布及表达。结果 随着肝纤维化的发展,大鼠肝纤维化组织中α-SMA阳性细胞明显增多(P<0.01),PTEN阳性表达细胞逐渐减少(P<0.01);PTEN蛋白在活化HSC广泛表达,主要表达于细胞质,随着肝纤维化的加重,表达PTEN的活化HSC占总的活化HSC的比例逐渐减少(P<0.01)。 结论 大鼠肝纤维化组织中PTEN阳性表达细胞减少,减少程度与HSC的活化、增殖呈负相关。

关键词: PTEN, 肝纤维化, 肝星状细胞

Abstract: Objective To explore the role of expression and distribution of PTEN protein in fibrotic liver tissue in the pathological process of hepatic fibrosis in rats. Methods The rat model of hepatic fibrosis was established by common bile duct ligation. The expressions and distribution of PTEN and α-SMA in rat liver tissues were detected by immunohistochemical staining, and expression and distribution of PTEN protein in activated hepatic stellate cell (HSC) in rat liver tissues was assayed by immunofluorescence double labeling confocal laser scanning microscopy. Results With the development of hepatic fibrosis, α-SMA positive cells in rat liver tissues increased significantly (P<0.01), while the expression of PTEN protein decreased gradually (P<0.01). PTEN protein expressed extensively in activated HSC in rat liver tissues, especially in the cytoplasm, and with the aggravation of hepatic fibrosis, the PTEN-expressing activated HSC accounted for an increasingly smaller percentage of total activated HSC (P<0.01). Conclusions The PTEN positive cells in rat liver tissues decreased, its dynamic expression has a significant negative correlation with the activation and proliferation of HSC in vivo.

Key words: PTEN, Hepatic Fibrosis, hepatic stellate cell