外周血miR-452、miR-221水平与经皮肾镜钬激光碎石术后并发尿源性脓毒血症的相关性

doi:10.16352/j.issn.1001-6325.2025.03.0298

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (3): 298-302.doi: 10.16352/j.issn.1001-6325.2025.03.0298

外周血miR-452、miR-221水平与经皮肾镜钬激光碎石术后并发尿源性脓毒血症的相关性

许伟杰, 陈家财, 赵力, 于忠英, 朱显钟, 李金雨^*

中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院泌尿外科,福建漳州 323000

收稿日期:2024-02-18 修回日期:2024-10-07 发布日期:2025-02-25
通讯作者: ^*lijinyu0920@163.com

Correlation of peripheral blood miR-452 and miR-221 levelswith urinary sepsis after percutaneous nephrolithotomy with holmium laser

XU Weijie, CHEN Jiacai, ZHAO Li, YU Zhongying, ZHU Xianzhong, LI Jinyu^*

Department of Urology, the 909th Hospital of the People′s Liberation Army of China/Dongnan Hospital of Xiamen University, Zhangzhou 323000, China

Received:2024-02-18 Revised:2024-10-07 Published:2025-02-25

摘要/Abstract

摘要： 目的探讨外周血miR-452、miR-221表达水平与经皮肾镜(钬激光)碎石术(PCNL)术后并发尿源性脓毒血症的相关性。方法选取2019年1月至2023年6月,于中国人民解放军联勤保障部队第909医院收治的92例PCNL术后并发尿源性脓毒血症患者作为疾病组,选择同期接诊的92例PCNL术后未并发尿源性脓毒血症患者作为对照组。用RT-qPCR检测外周血miR-452、miR-221表达水平;用Logistic回归分析PCNL术后并发尿源性脓毒血症的影响因素;用受试者工作特征(ROC)曲线评估外周血miR-452、miR-221水平对PCNL术后并发尿源性脓毒血症的诊断效能。结果疾病组患者手术时间长于对照组,外周血miR-452、miR-221水平均高于对照组(P＜0.05)。外周血miR-452、miR-221、手术时间、降钙素原(PCT)、C反应蛋白(CRP)、中性粒细胞与淋巴细胞比例(NLR)、尿常规是影响PCNL术后并发尿源性脓毒血症的影响因素(P＜0.05)。外周血miR-452、miR-221联合诊断患者PCNL术后并发尿源性脓毒血症的曲线下面积(AUC)为0.888,均优于各自单独检测(Z二者联合-miR-452=2.005、Z二者联合-miR-221=2.972,P＜0.05、0.01),敏感度与特异度分别为77.17%、91.30%。结论外周血miR-452、miR-221水平变化与PCNL术后并发尿源性脓毒血症密切相关,联合检测对PCNL术后并发尿源性脓毒血症有较高的诊断效能。

关键词: 钬激光, 经皮肾镜碎石术, miR-452, miR-221, 尿源性脓毒血症

Abstract: Objective To explore the correlation between the expression of microRNA-452 (miR-452) and micro-RNA-221 (miR-221) in peripheral blood and post-operative urinary sepsis after percutaneous nephrolithotripsy (PCNL). Methods From January 2019 to June 2023, 92 patients with post-operative urinary sepsis after PCNL admitted to 909 Hospital of the Chinese People′s Liberation Army Joint Logistic Support Force were regarded as the disease group, 92 patients who underwent PCNL surgery during the same period without urinary sepsis were collected as control group. RT-qPCR was applied to detect the expression of miR-452 and miR-221 in peripheral blood; Logistic regression was applied to analyze the influencing factors of urinary sepsis after PCNL surgery; Receiver operating characteristic (ROC) curve was applied to evaluate the diagnostic efficacy of peripheral blood miR-452 andmiR-221 levels for post-operative urinary sepsis in PCNL patients. Results The time length of the surgical operation in disease group was longer than that in the control group, and the level of miR-452 and miR-221 in peripheral blood, were higher than those in the control group (P＜0.05). Peripheral blood miR-452, miR-221, procalcitonin (PCT), C-reactive protein (CRP), neutrophil to lymphocyte ratio (NLR), urine routine and surgical time were all influence factors for post-operative urinary sepsis in PCNL (P＜0.05). The area under the curve (AUC) for the combined diagnosis of peripheral blood miR-452 and miR-221 in patients with urinary sepsis after PCNL surgery was 0.888, which was better than their individual detection (Z_{combination-miR-452}=2.005, Z_{combination-miR-221}=2.972, P=0.045, 0.003), the sensitivity and specificity were 77.17% and 91.30%, respectively. Conclusions The change of miR-452 and miR-221 level in peripheral blood is closely related to urinary sepsis after PCNL. Combined testing has a high diagnostic efficacy for post-operative urinary sepsis after PCNL surgery.

Key words: holmium laser, percutaneous nephrolithotomy, miR-452, miR-221, urogenic sepsis

中图分类号:

R692

许伟杰, 陈家财, 赵力, 于忠英, 朱显钟, 李金雨. 外周血miR-452、miR-221水平与经皮肾镜钬激光碎石术后并发尿源性脓毒血症的相关性[J]. 基础医学与临床, 2025, 45(3): 298-302.

XU Weijie, CHEN Jiacai, ZHAO Li, YU Zhongying, ZHU Xianzhong, LI Jinyu. Correlation of peripheral blood miR-452 and miR-221 levelswith urinary sepsis after percutaneous nephrolithotomy with holmium laser[J]. Basic & Clinical Medicine, 2025, 45(3): 298-302.

参考文献

[1] Massella V, Pietropaolo A, Gauhar V, et al. Has fluoroless endourology (URS and PCNL) come of age? evidence from a comprehensive literature review[J]. Actas Urol Esp (Engl Ed), 2023, 16:2173-5786.
[2] Atassi N, Knoll T. Future of kidney stone management: surgical intervention miniaturization of PCNL: Where is the limit?[J] Curr Opin Urol, 2020, 30:107-112.
[3] Sigurdarson JJ, Svane S, Karring H. Development of a M9-based urea medium (M9U) for sensitive and real-time monitoring of ureolytic activity of bacteria and cell-free urease[J]. Microbiologyopen, 2020, 9:e976. doi: 10.1002/mbo3.976.
[4] Cron MA, Guillochon É, Kusner L, et al. Role of miRNAs in normal andmyasthenia gravis thymus[J]. Front Immunol, 2020, 11:1074.doi: 10.3389/fimmu.2020.01074.
[5] Di Martino MT, Arbitrio M, Caracciolo D, et al. miR-221/222 as biomarkers and targets for therapeutic intervention on cancer and other diseases: a systematic review[J]. Mol Ther Nucleic Acids, 2022, 27:1191-1224.
[6] 叶章群. 泌尿外科疾病诊疗指南[M]. 北京:科学出版社, 2013:78-79.
[7] 李晓慧, 岳胜, 杜小静, 等. ICU重症感染患者血清C-反应蛋白、内毒素、肝素结合蛋白水平及其预测价值[J]. 中华医院感染学杂志, 2020, 30:2647-2651.
[8] 查娜,郝丹丹,李天柱.MicroRNAs通过Hippo通路抑制肝细胞癌发生的研究进展[J]. 基础医学与临床,2023,43:1871-1875.
[9] Liu Z, Yang D, Gao J, et al. Discovery and validation of miR-452 as an effective biomarker for acute kidney injury in sepsis[J]. Theranostics, 2020, 10:11963-11975.
[10] Markovic J, Sharma AD, Balakrishnan A. MicroRNA-221: a fine tuner and potential biomarker of chronic liver injury[J]. Cells, 2020, 9:1767.doi: 10.3390/cells9081767.
[11] Gaytán-Pacheco N, Ibáñez-Salazar A, Herrera-Van Oostdam AS, et al. miR-146a, miR-221, and miR-155 are involved in inflammatory immune response in severe COVID-19 patients[J]. Diagnostics (Basel), 2022, 13:133. doi: 10.3390/diagnostics13010133.
[12] Shi B, Zhang J, Chen Q. Liver and kidney surgical anatomy to verify the effect of miR-221 on organ damage in septic rats[J]. J Healthc Eng, 2022, 2022:2814431.doi: 10.1155/2022/2814431.

编辑推荐 0

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	0	0	0	20

	来源	本网站

	次数	20
	比例	100%

摘要

最新录用	在线预览	正式出版

0	0	49

	来源	本网站

	次数	49
	比例	100%

外周血miR-452、miR-221水平与经皮肾镜钬激光碎石术后并发尿源性脓毒血症的相关性

Correlation of peripheral blood miR-452 and miR-221 levelswith urinary sepsis after percutaneous nephrolithotomy with holmium laser

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐 0

Metrics

本文评价