Abstract: Objective To study the effect of PcG member NSPc1 on the proliferation of HeLa cells. Methods Using bioinfomatic analysis to design the siRNA sequence for knockdown NSPc1. Detecting the expression level of NSPc1 in HeLa cell lines using semi-quantitative RT-PCR, Real-time PCR and Western blot after transfection of the designed siRNA. Transient transfecting pSUPER-NSPc1 into Hela cells and performing BrdU incorporation assay. Establishing NSPc1 stably knockdown cell lines, comparing proliferation abilities with the control cells. Results 1) The designed siRNA could efficiently knockdown the expression of NSPc1; 2) Transient knockdown NSPc1 could repress BrdU incorporation; 3) The established NSPc1-knockdown cell lines had a significantly low proliferation rate than control cells. Conclusion The expression of NSPc1 is necessary for the normal proliferation of HeLa cells.The NSPc1 stably knockdown cell pools is a useful model for further study the pathway related to NSPc1.

Key words: Polycomb, NSPc1, HeLa cell, Stable cell line, Proliferation