Abstract: Objective To investigate expression changes of cofilin-1 in cell BGC-823 treated with ursolic acid. Methods Cell BGC-823 was treated with ursolic acid, expression changes of cofilin-1 was detected by 2-D-electrophoresis and mass spectrum, protein expression of cofilin-1 and cytochrome c was detected by western blot. Results Lower expression of cofilin-1 in cytoplasm(p<0.01), but obvious raise in mitochondria(p<0.01), and down-regulation of cytochrome c in cytoplasm(p<0.01). Conclusions BGC-823 was treated with ursolic acid，might made cofilin-1 take transposition from cytoplasm to mitochondria，which promoted cytochrome c to release from mitochondria，activated caspase cascade，and induced cell apoptosis.