Abstract: Objective To prepare Rabbit anti-pig plasma Fibronectin (FN) serum and to explore the effect of different immune approaches, the number of immunization and species of rabbits on the antibody titer. Methods Inbred New Zealand rabbits and cross bred rabbits respectively were inoculated by different programs which were hypodermic injection of 1 plus lymph nodethen injection of 5 or 3 times , intramuscular injection of 1 plus intravenous injection of 5 or 3 times with porcine plasma FN. Then, FN antibody was determined by the method of double immundiffusion. Results New Zealand rabbits and cross bred rabbits could bring anti-FN by hypodermic injection plus lymph nodethen injection or intramuscular injection plus intravenous injection. In addition, the antibody titer of the former was obviously higher than that of the latter and 5 times intensive immunization was higher than 3 times intensive immunization. Conclusions To obtain Rabbit anti-pig plasma FN serum, it may be better to inoculate rabbits through hypodermic injection plus lymph nodethen injection and multiple immunization．Moreover, inbred New Zealand rabbits were easy to induce anti-FN than cross bred rabbits .

Key words: FN, Antiserum, Preparation, Route of immunization