Abstract: Objective To investigate the effects of progesterone on apoptosis and surviving expression protein of Ishikawa cell line of human endometrial carcinoma in vitro, Methods The proliferational of Ishikawa cells cultured in vitro were determined by methyl thiazolyl terazolium (MTT) medhod.Cell cycle, apoptotic percentage were detected by cytometer . The expressions of survivin protein of Ishikawa cells were detected by immunocytochemistry. Results progesterone inhibited the growth of Ishikawa cells significantly when the concentration of progesterone was at 0.5×10-5~10-4mol/l(p<0.01). Flow cytometry(FCM) showed that the proportion percentage of G0/G1 phase and the apoptotic rate were increased significantly. The proportion of S phase was reduced (p<0.01). Apoptosis morphology was observed in each group . The expression of survivin of in Ishikawa cells was lowed after the cells was treated with P. Conclusion Progesterone could inhibit the proliferation and induce apoptosis of Ishikawa cells. The regulation on the expression of the survivin protein by progesterone seems to be responsible for the effects on the apoptosis in Ishikawa cells.

Key words: Endometrial neoplasm, Progesterone, Proliferation, Apoptosis