Basic & Clinical Medicine ›› 2009, Vol. 29 ›› Issue (6): 631-635.

• 临床园地 • Previous Articles     Next Articles

Evaluation of several Dermatophagoides pterronyssinus Allergen extracts for Skin Prick Test

Jin-lu SUN, Rui-qi WANG, Jia YIN, Liang-lu WANG, Hong-yu ZHANG, Hong LI, Li-ping WEN   

  1. Peking Union Medical College Hospital, CAMS & PUMC
  • Received:2009-03-24 Revised:2009-04-29 Online:2009-06-25 Published:2009-06-25
  • Contact: Jin-lu SUN,

Abstract: Objective To evaluate the significance of several different house dust mite allergen extracts with skin prick test (SPT) in allergic patients to Dermatophagoides pteronyssinus. Methods 219 patients underwent SPT and serum specific IgE assay to house dust mite. 3 kinds of house dust mite allergen extracts were used for SPT, respectively. Extract A group was prepared by our laboratory, extract B group was extract of Dermatophagoides pteronyssinus manufactured by ALK Corporation, and extract C group was mixed extract of Dermatophagoides pteronyssinus and Dermatophagoides farinae manufactured by ALK Corporation. Human serum specific IgE was regarded as reference of allergy to Dermatophagoides pteronyssinus. Evaluate the ROC curves of these three groups. Results 1. The medium of wheal diameter of SPT with A group, B group and C group was 0.45cm, 0.35cm and 0.30cm, respectively. 2. There was no local urticaria and systemic urticaria, no running nose and sneezing, no cough and asthma, no allergic shock observed. Local reactions after SPT were observed in 5 ( group A), 3 ( group B), 3 patients ( group C).. The delayed reactions were observed in 2 patients of each group. The area under ROC curve of SPT with extract A group, B group and C group was 0.765、0.801、0.782. The coefficient of linear correlation was 0.771, 0.786 and 0.784, respectively (P<0.01), the coefficient was statistically significant. 4. Based on serum specific IgE, the sensitivity of SPT with extract A group, B group and C group was 92.4%, 87.0% and 81.5%, and the specificity was 60.6%, 73.2% and 74.8%, respectively. Conclusion The house dust mite allergen extract prepared in our laboratory was a proper extract for diagnosing of house dust mite allergy. The sensitivity and specificity of the extract was good, and it can be used in clinical diagnosis.

Key words: Dermatophagoides pteronyssinus, allergen, extract, ROC curve