Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (3): 368-373.

• Original Articles • Previous Articles     Next Articles

rL-IL29 inhibits proliferation and migration of human gastric cancer cell line BGC

YIN Chao-yun1, ZHANG Yao2, CHEN Zhen-wei2, ZHANG An-wei2, ZHANG Xuan-feng2, ZHANG Ri-ting2, BU Xue-feng3*   

  1. 1. Department of Vascular Surgery,the Affiliated Hospital of Jiangsu University,Zhenjiang 212000;
    2. School of Medicine,Jiangsu University, Zhenjiang 212013;
    3. Department of General Surgery,the Affiliated People's Hospital of Jiangsu University,Zhenjiang 212002,China
  • Received:2019-03-08 Revised:2019-09-25 Online:2020-03-05 Published:2020-03-02
  • Contact: *

Abstract: Objective To investigate the effect of recombinant Newcastle disease virus expressing IL-29(rL-IL29) on the proliferation and migration with invasion of gastric cancer cells and to explore the potential mechanism. Methods Gastric cancer cell lines BGC were cultured and divided into the PBS, rL-IL29 and newcastle disease virus(NDV)groups infected by the PBS, rL-IL29 or NDV.Western blot was used to detecte the expression of NDV, IL-29,p-ERK,MMP2 and p-AKT protein in cells.The proliferation,migration and invasion of gastric cancer cell lines BGC were detected by CCK8、cell clone forming experiments、scratch test and Transwell invasion test. Results IL-29 protein was only expressed in rL-IL29 group,NDV protein were expressed both in rL-IL29 group and NDV group and was significantly higher than those of PBS group (P<0.05). The proliferation and migration of BGC cells infected with rL-IL29 were inhibited more seriously than those infected with NDV or PBS group.The expression of p-ERK,MMP2 (66 ku/72 ku),p-AKT and P65 protein was all significantly decreased(P<0.05). Conclusions The proliferation and migration of gastric cancer cell lines BGC were inhibited by rL-IL29 and the mechanism may be related with ERK,AKT signaling pathways.

Key words: recombinant Newcastle disease virus, IL-29, gastric cancer cell line BGC, proliferation, migration

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