Abstract: Objective To analyze the effect of ATAD2 on glioma cell lines. Methods ATAD2 levels in U87MG, U251 and normal human astrocytes were detected by real-time PCR and Western blot. U87MG and U251 cells were divided into four groups: control, mock (lipofection control), ATAD2 siRNA (transfected with ATAD2 siRNA) and ATAD2 overexpression (transfected with ATAD2). Cell proliferation, migration and invasion were respectively measured by MTT and Transwell assay. The levels of E-cadherin, N-cadherin and Vimentin were measured by Western blot. Results ATAD2 was highly expressed in U87MG and U251 cells. Compared with control, cell proliferation, invasion, migration, N-cadherin and Vimentin expression were decreased by ATAD2 siRNA, while they were promoted by ATAD2 overexpression (P<0.05). E-cadherin expression was upregulated by ATAD2 siRNA and it was inhibited by ATAD2 overexpression (P<0.05). Conclusion ATAD2 participants in human glioma cells metastasis via epithelial-mesenchymal transition (EMT).

Key words: invasive power, neuron