Basic & Clinical Medicine ›› 2014, Vol. 34 ›› Issue (4): 527-530.

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The value of T-SPOT.TB in the differential diagnosis of Sarcoidosis and Pulmonary Tuberculosis


  • Received:2013-04-01 Revised:2013-10-28 Online:2014-04-05 Published:2014-03-31

Abstract: Objective T-SPOT.TB is a new method to diagnose tuberculous infection through detecting the frequencies of IFN-γ producing T cells which respond to the M.tuberculosis specific antigens encoded by the RD1 region(ESAT-6 and CFP-10). This study aimed to evaluate the role of T-SPOT.TB in the differentiation of the sarcoidosis and tuberculosis. Methods The data of T-SPOT.TB of 44 sarcoidosis and 30 pulmonary tuberculosis patients in PUMCH between January 2008 and March 2011 were analysed. Results Among the 44 sarcoidosis patients ,the T-SPOT.TB result was positive in 12 patient (27.3%) ,while among the 30 pulmonary tuberculosis patients ,the T-SPOT.TB result was positive in 27 patient (90%) .Pooled frequencies of ESAT-6 and CFP-10 specific IFN-γ secreting T cell was 46SFCs/106PBMC(IQR 28~204) for sarcoidosis patients ,significantly lower than that for pulmonary tuberculosis patients which was 565 SFCs/106PBMC(IQR 170~2340). Conclusion T-SPOT.TB may be a new method for the differentiation of sarcoidosis from pulmonary tuberculosis.

Key words: [Key words]:Sarcoidosis, Pulmonary tuberculosis, Differential diagnosis, ELISPOT, T-SPOT.TB