Table of Content

05 April 2014, Volume 34 Issue 4

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The gene expression profile analysis of regDC induced by MEF-MSC

2014, 34(4):  433-438. 

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Objective The aim is to investigate the difference of gene expression between MEF-regDC and imDC & maDC, in order to demonstrate the character of MEF-regDC. Methods We first harvest MEF-regDC by co-culture of bone marrow hematopoietic stem cell with MSC, then analysis 3 types of DCs, including Fold change, Principal Component Analysis and GoTerm to Gene. Results MEF-regDC has the characteristic of morphology, phenotypes and gene expression profile, the PCA illustrate that MEF-regDC and imDC & maDC are 3 different type of DC, and there are 13369 genes that are 2 folds different between MEF-regDC and imDC, and the number of genes that are 6 folds different between the 2 types of DCs is 3247; meanwhile, the number of different genes between MEF-regDC and maDC are 11326 and 3299, respectively. Further analysis demonstrate that, there are obvious difference of function regulate genes, immune-related genes and signaling pathways related genes among these three type of DCs. Conclusion MEF-regDC is a new type of DC which is different from imDC and maDC, and has characteristic gene expression profiles.

Knocking out human PDE10A gene by the CRISPR/Cas9 system

2014, 34(4):  439-443. 

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Objective To construct the CRISPR/Cas9 system of knocking out the PDE10A gene. Methods Three 20 bp sgRNAs targeting exon 6, exon 7 and exon 11 of PDE10A were designed, chemically synthesized, and then inserted into linearized plasmid, PX330. Next, the correct PX330-sgRNA plasmids were transfected into HEK293T cells after verification by Sanger sequencing. The targeting efficiency was detected by SURVEYOR assay and the nicked site was further detected by Sanger sequencing. Finally, the stable PDE10A knockout 293T cell lines were selected by the limiting dilution method. Results The target nucleotide sequences were successfully inserted into the expected sites of vector and sequences were correct. The targeted exon 7 sgRNA can successfully knock PDE10A, and the targeting efficiency was up to 31.4 %. Stable knockout PDE10A cell line was selected successfully which harbored 2 bp deletion. Conclusion The CRISPR/Cas9 system of knocking out the PDE10A gene was successfully constructed.

Hesperidin pretreatment attenuates concanavalin A-induced acute liver injury

2014, 34(4):  444-448. 

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Objective To investigate the protective effects of hesperidin (HDN) on hepatic injury induced by concanavalin A (Con A) in mice. Methods Hesperidin was administrated to mice daily for ten days via gastric gavage and then acute liver injury model was established by intravenous administration of Con A. Liver and spleen were weighed to calculate liver and spleen index. ALT and AST in serum, MDA and SOD was measured by reverse transcription polymerase chain reaction (RT-PCR). Results HDN pretreatment significantly decreased liver and spleen index, serum levels of ALT and AST (P＜0.01); pathological changes in hepatic tissue were dramatically improved; the content of MDA was decreased and the activity of SOD was increased (P＜0.01); the extent of hepatic apoptosis was significantly reduced; more importanty, Bcl-2 mRNA expression was up-regulated while Bax mRNA expression was down-regulated markedly (P＜0.01). Conclusion HDN pretreatment protects mice from Con A-induced acute liver injury probably through inhibiting lipid peroxidation and hepatocyte apoptosis.

The role of Pak1 in spindle formation of mouse oocytes

2014, 34(4):  449-453. 

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Objective: To investigate the suncellular localization of autophosphorylated Pak1 in mouse oocytes during meiosis and its correlation with spindle formation. Methods: Western blot procedure was employed to assay the dynamic changes in protein expression of autophosphated Pak1 at Ser144(pPak1S144); immunofluorescence staining was carried out to label pPak1S144 subcellular distribution and its temporal-spatial relationship with spindle and microtubule organizing centers(MTOCs) proteins, Pericentrin and ?-tubulin. Results: pPak1S144 is co-localized with Pericentrin and ?-tubulin and concentrated on spindle poles. Conclusion: pPak1S144 is MTOC-associated protein, implying a role in meiotic spindle formation.

Genotoxic effect of formaldehyde on mouse BM-MSCs

2014, 34(4):  454-458. 

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Objective Study the genotoxic effect of formaldehyde on BM-MSCs, thus will provides the biological evidence for the formaldehyde to lead to leukemia. Methods Proliferation activity was measured by MTT assay; Genotoxic effect was detected by comet assay, KCl-SDS precipitation assay, sister chromatid exchange (SCE) test and micronucleus (MN) test. Results 75~200 μmol/L formaldehyde can inhibit the BM-MSCs proliferation in a dose-dependent manner (P<0.01); DNA strand breakage increased gradually at increasing concentrations below 125 μmol/L, however, when formaldehyde concentration was over 125 μmol/L, DNA strand breakage decreased. There were significant rises in 75~150 μmol/L formaldehyde groups compared with control group (P<0.01); Induction of DNA-protein crosslinks (DPCs), SCE and MN in ≥125 μmol/L formaldehyde groups were significant higher than in control group (P<0.01). Conclusion Formaldehyde can inhibite the BM-MSCs proliferation activity, and have a genotoxic effect on the BM - MSCs.

The molecular mechanisms of human gastric cancer cell line MGC-803 proliferation suppressed by overexpression of E2F-1 mediated by lentivirus

2014, 34(4):  459-463. 

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Objective To study the molecular mechanisms of human gastric cancer cell line MGC-803 proliferation suppressed by overexpression of E2F-1 . Methods The experimental group(LV-E2F-1-GFP group ),gastric cancer MGC-803 cells were transfected with the recombinant lentivirirus vector (LV-E2F-1-GFP) ,the negative control group were transfected with the negative control lentiviral vector (LV- GFP),the blank control group was untreated. The expression of Skp2, Bax, Bcl-2 and Survivin were detected by semi-quantitative PT-PCR and Western blot method . Results Compared with LV- GFP group and blank control group,the mRNA levels of Skp2, Bcl-2 and Survivin in the LV-E2F-1-GFP group were reduced(P<0.05),the protein levels of Skp2, Bcl-2 and Survivin in the LV-E2F-1-GFP group were reduced(P<0.05), the mRNA level of Bax and the protein level of Bax were up regulated (P<0.05). Conclusion The proliferation of human gastric cancer MGC-803 cells was inhibited by overexpression of E2F-1 mediated by lentivirus, the effect may be related with down-regulation of Skp2,Survivin,Bcl-2 expression and up-regulation of Bax expression.

Exenatide Treatment Improves Nonalcoholic Fatty Liver Phenotype by Regulation of PPARα and ACOX1

2014, 34(4):  464-469. 

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Objective To observe the Exenatide effect on fat-induced nonalcoholic fatty liver disease (NAFLD) in rat. Methods 120 SD rats were randomly divided into control group( CON), model group(HFD), Exenatide low, medium, high dose group ( ELD, EMD, EHD) and polyene phosphatidylcholine treatment group (PDC), each group was 20. After successfully established NAFLD, given appropriate treatment, half of the rats were sacrificed after 4 and 8 weeks. HE staining of liver slices, the corresponding detection kit liver function, blood lipids function, RT-PCR and western blot measured PPARα and ACOX1 expression. Results After 8 weeks of treatment, rat liver HE staining PDC, EMD and EHD have not any fat particles infiltration; after 4 weeks treatment, liver function AST, ALT and CHE, serum lipids CHOL and TG compared with HFD group, PDC, ELD, EMD and EHD, p <0.05,8 weeks further reduced; liver Peroxisome Proliferator-Activated Receptor α (PPARα) and Acyl Coenzyme A Oxidase 1(ACOX1) expression has also undergone a significant improvement. Conclusion Exenatide improves NAFLD Phenotype by regulation of PPARα and ACOX1 expression.

The Influence and mechanism of the NOK oncogene on the G1/S Distribution of human embro kidney 293T Cells

2014, 34(4):  470-474. 

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Objective To analyze the influence of the NOK oncogene on the G1/S distribution in 293T cells and to elucidate the regulated mechanisms responsible for NOK mediated G1/S phase control. Methods, After transiently transfecting NOK into 293T, 293ET and HeLa cells, we detected the cell cycle distributions by flow cytometric approach. Then, we detected the protein expressions of G1/S check point related genes such as cyclin D, cyclin E1,CDK2 and CDK4 by western blot analysis. Results In the 293T、293ET and HeLa cell which was transfered by NOK, the G1 phase proportions of cells were reduced, but the S phase proportions of cells were increased (p<0.05). Significant up-regulations in the expressions of cyclin D,cyclin E1, p-CDK-2, p-CDK-4, p-Akt and p-Rb were observed with the increased delivery of NOK into 293ET cells. Conclusion NOK can promote cell cycle progression by rapidly passing the G1/S check point, which may involve the activations of both Akt and CDK2/4 signaling pathways.

Comparison of clinic applications in malignant tumor immunotherapy between autologous and healthy CIK cells

2014, 34(4):  475-479. 

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Objective To compare the clinical anti-tumor effect of cytokine-induced killer cells(CIK) derived from patients with malignancies or healthy doners. Methods Peripheral blood mononuclear cells(PBMC) of patients(n=12) or healthy doners(n=12) were cultured in presence of IFN-γ, CD3 Ab, IL-1 and IL-2. The proliferation was counted by Trypan blue test, the in vitro anti-tumor cytotoxicity was analysed by CCK-8 assay, the phenotypes were analyzed by flow cytometry, the duration of healthy CIK was determined by PCR, the clinical effects were compared finally. Results Compared with autologous CIK, healthy CIK showed higher proliferative ability and enhanced in vitro anti-tumor cytotoxicity(P<0.05), and led to an efficient recovery of immunologic function of recipients. 5～8w after infusion, healthy CIK could also be detected in recipients’ peripheral blood. More importantly, healthy CIK showed equivalent therapeutic effect and minimal adverse reactions. Conclusion Application of healthy CIK cells in treatment of malignancies is efficient and feasible, which would gain a better clinic application prospect.

Overexpression of GBA induces autophagy and decreases the expression level of α-synuclein in SH-SY5Y cells

2014, 34(4):  480-484. 

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Objective To investigate the mechanism of autophagy induced by GBA and the regulation of GBA on the expression of α-synuclein in SH-SY5Y cells. Methods The recombinated plamid EGFP-N1, EGFP-GBA were transfected into SH-SY5Y cells through eukaryotic cell transfection technique. The expression of beclin1, LC3-II/LC3-I and α-synuclein in the level of protein was detected through Western blot，MDC staining and cellular immunofluorescence microscopy were performed to observe the change of autophagic vacuolization and their subcellular colocalization. Results Compared to EGFP-N1 group, the expression of Beclin1 and LC3-II/LC3-I in group of overexpression EGFP-GBA were significantly increased, and the average number of autophagic vacuoles per cell increased, however, the expression of α-synuclein was significantly decreased (P<0.05) . Besides, the signals for LC3, GBA and the extent of their co-localization increased in GBA groups. Conclusion GBA protein decreases the expression level of α-synuclein and induces autophagy in SH-SY5Y cells.

On Spectrum analysis of Enteroviruses in Children with Acute Respiratory Tract Infections in Beijing

2014, 34(4):  485-489. 

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Objective To determine the spectrum of enteroviruses (EV) in children with acute respiratory tract infections (ARTI) in Beijing. Method Throat swabs were collected from 1,121 children at the time of admission to the Beijing Children’s Hospital for ARTI during May 2009 through September 2009. To detect EV, the viral protein 1 (VP1) gene were amplified by reverse transcription PCR and verified the findings by sequence analysis. Results EV was detected in 55 children and 14 EV serotypes were identified. Among of them, 7 EV serotypes including 44 cases belonged to EV-A and the others belonged to EV-B. The sequences of EV in this research had very high homology with that detected in hand-foot-and-mouth disease or aseptic meningitis. Compared to prototype, mutations were happened in clinical strains. Conclusions EV-A was dominant in children with acute respiratory tract infections which was different to that of the adult in Beijing in 2009.

Investigation of job satisfaction and training needs for the staff in Daqing Community Health Center

2014, 34(4):  490-493. 

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Objective To investigate personnel structure, staff’s job satisfaction and training needs at two community health centers in Daqing city. Methods A questionnaire-based survey was conducted among staff members in JY and YW community health centers in Daqing city. Results Forty-nine participants (92.5%) completed the survey. More than 90% of the respondents have received at least one time of continuing education which was mainly pertaining to medical or nursing techniques. However, more than half of the participants reported that training in health education and psychology were most needed currently for their professional capacity. More than 95% of the respondents were satisfied with the doctor/nurse-patient relationship; about 84% were satisfied with well-established standards for their practice and the supportive policies offered by government for the community health centers. Nevertheless nearly half of the respondents were not satisfied with resources of equipment and staffing, salaries and staff benefits. Compared to nurse, fewer physician and administrative staff expressed satisfaction. Staff working in the communities longer was less satisfied with the working environment than those working shorter. Conclusion Health education and psychology should be emphasized in continuing education for staff in community health centers. Improvement in benefits may improve staff’s job satisfaction.

Construction, packaging and expression activity detecting of miR-30a expression vector

2014, 34(4):  494-498. 

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Objective: To construct lentiviral miR-30a expression vector for the study of the mechanism of miR-30a in chronic myeloid leukemia and the impact to imatinib resistance. Methods: Human bone marrow genomic DNA was extracted, miR-30a precursor sequence was amplificated and cloned into the lentivirus expression vector plVTHM, then sended sequencing. The recombinant vector was packaged in 293T cells and tittered in K562 cells. GFP-positive K562 cells with green fluorescence were sorted by flow cytometry, miR-30a expression levels in sorted cells were further detected using real-time quantitative PCR. The proliferation of different cells was detected by CCK-8 assay. Results: Sequencing of the recombinant lentiviral expression vector was completely correct; viral titer was 6 × 106TU/mL; 160μL virus solution / 1mL medium was selected to infect K562 cells; the expression level of miR-30a in infected K562 cells was significantly enhanced(P<0.05); the proliferation levels of cells overexpressed miR-30a decreased significantly(P<0.05). Conclusion: MiR-30a lentivirus vector was successfully constructed and miR-30a could be stably expressed in K562 cells, and miR-30a could inhibit the proliferation level of K562 cells.

Expression increased of CXCR4 and MMP-2 in clear cell renal cell carcinoma

2014, 34(4):  499-503. 

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Objective To study the expression and significance of CXC chemokine receptor 4（CXCR4）and matrix metalloproteinases-2（MMP-2）in clear cell renal cell carcinoma（ccRCC）tissues.Methods The expression of CXCR4 and MMP-2 mRNA in 88 cases of fresh ccRCC tissues and 48 cases of normal renal tissues were detected by RT-PCR. Meanwhile, the expression of CXCR4 and MMP-2 protein were examined by immunohistochemical PV6000 assay.Results The expression of CXCR4 and MMP-2 mRNA and protein in ccRCC tissues were significantly higher than those in normal ones（P<0.05）. Furthermore, the expression of CXCR4 and MMP-2 in ccRCC tissues had a significant relationship with clinical stage, pathological grade and lymph node metastasis（P<0.05）, while not with gender and age of patients and the diameter of tumors（P>0.05）. Moreover, there was a positive correlation between the expression of CXCR4 and MMP-2 in ccRCC tissues （P<0.05）. Conclusion CXCR4 and MMP-2 may have a synergistic effect in the process of occurrence,development, invasion and metastasis of ccRCC,they may provide a novel strategy for diagnosis and therapy of ccRCC.

Matrine alleviates Blemycin-induced pulmonary fibrosis in rats

2014, 34(4):  504-508. 

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Objective To discuss the molecular mechanism and protective effect of Matrine on Blemycin-induced pulmonary fibrosis rat. Methods Rats were randomly divided into five groups, the control group, model group, Prednisone, Matrine low dose and high dose group.Pulmonary fibrosis model was made by adminstrated blecomin (5 mg/kg) through trachea. From 1st day after modeling, the rats were fed prednisone acetate (0.56mg/kg/d) or different dose matrine (50 and 100mg/kg/d). At the 7, 14, 28 days of modeling, the lung tissue biopsy sections are observed by HE and Masson staining pathology. The malondialdehyde (MDA) level and hydroxyproline (HYP) content in lung tissue was detected, and HO-1 mRNA was measured by RT-PCR, and production of TNF-α. The lung tissue biopsy sections are detected by HE and Masson staining pathology. Results In model group, inflammatory response was the main performance at 7d. At 28d, more lung fibroblast could be found. The collagen could be found more clearly, as demonstrated by Masson staining. Pathological changes in the in the matrine and prednisone treated group were less than the model group. The MDA and HYP content in model group was higher compared with normal control group (p<0.05), and decreased after matrine and prednisone treatment in 28d (p＜0.05). In the normal lung tissue, expression of HO-1 and production of TNF-αwere very low, and increased in the model group. After drug intervention, the levels of both TNF-α and HO-1 were decreased (p<0.05). Conclussions Matrine can alleviate the fibrosis status in rats, and the mechanism may be associate to regulate oxidative-antioxidant imbalance, and inhibition of TNF-α and HO-1 expression.

Association between Metabolic Syndrome and Nonalcoholic Fatty Liver Disease in 6 660 physical examination population of Guilin

2014, 34(4):  509-513. 

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Objective To investigate association between metabolic syndrome(MS) and nonalcoholic fatty liver disease(NAFLD) in physical examination population of Guilin. Methods 6 660 persons were selected from medical center for health examination by cluster sampling method. The survey included questionnaire investigation, physical examination, metabolic characteristics and liver color doppler ultrasound examination, and body mass index(BMI) calculated. Results 1) Among the 6 660 participants, the prevalence of NAFLD、MS and (NAFLD+MS)were 19.1%、11.4% and 6.2%. The prevalence of NAFLD、MS and (NAFLD+MS) increased with age, the prevalence of age-stratified and gender-stratified were significant differences (P<0.01). 2) There were all significant differences of each component of MS in comparison of NAFLD and control group (P<0.01). 3) BMI、systolic blood pressure (SBP)、diastolic blood pressure (DBP)、fasting blood glucose(FPG)、triglyceride(TG)、total cholesterol(TC)、Low-density lipoprotein cholesterol(LDL-C)、alanine aminotransferase(ALT)、aspartate aminotransferase(AST) and uric acid(UA) in NAFLD group were significantly higher than in normal control group (P<0.01), high-density lipoprotein cholesterol(HDL-C) level was lower in NAFLD group than in normal control group (P<0.01). 4) Logistic regression anaIysis demonstrated that sex(male)、BMI、blood pressure、FPG、TG、LDL-C、ALT and UA were the risk factors of NAFLD, and HDL-C was the protective factor of NAFLD. Conclusions NAFLD was positively correlated with MS, and had the characteristics of each component of MS.

Ulinastatin alleviates patients’ inflammatory response, acute and chronic pain after bilateral total knee arthroplasty

2014, 34(4):  514-518. 

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Objective To investigate the effect of ulinastatin (Urinary Trypsin Inhibitor, UTI) on patients’ perioperative inflammatory response, postoperative acute and chronic pain, and the rehabilitation of knee joint after bilateral total knee arthroplasty (TKA) under pneumatic tourniquet. Methods 40 patients scheduled for bilateral TKA under thigh tourniquet, were enrolled and randomly assigned to trial group (U group) and control group (C group). U group received intravenous ulinastatin and C group received saline at identical time points. All patients received the same general anesthesia, postoperative analgesia and rehabilitation protocols. The plasma concentration of inflammatory cytokines, such as IL-6, TNF-α, and IL-10, were recorded at different time points. The patient's VAS at rest and activity, the opioid consumption, the range of active knee flexion and continuous passive motion, and the time of walking 30 meters were observed and compared. Results No significant differences were found between two groups regarding the demographic data. The inflammatory response of U group was lower than C group. The VAS at rest and activity and sufentanil consumption at 4 hours after the surgery of U group were significantly lower than C group. No statistically significant difference was found in postoperative functional rehabilitation parameters at all time points. Conclusions Ulinastatin can alleviate perioperative inflammatory response, decrease early postoperative resting pain scores and reduce opioid consumption.

Ondansetron added to intravenous morphine analgesia pump could not reduce postoperative nausea and vomiting in female patients

2014, 34(4):  519-522. 

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Objective To evaluate whether ondansetron added to intravenous patient analgesia solution with morphine will reduce postoperative nausea and vomiting. Methods Data of surgical female inpatients in PUMCH from January 1st to June 30th, 2012 who performed general anesthesia and intravenous analgesia solution with morphine postoperatively were analyzed. Results Data from 578 cases were analyzed. Cases of no adding ondansetron to intravenous analgesia solution were 55, compared to adding ondansetron group were 523 (ondansetron 0.2mg~0.5mg/h for 48 hours). The maximum VAS score of postoperative pain at rest, the maximum VAS score of postoperative pain during movement and postoperative sedation scores were no significantly different. The maximum score of postoperative nausea, and cumulated frequency of postoperative vomiting during first 48 hours were no significantly different. Conclusion Ondansetron added to intravenous patient-control analgesia solution with morphine could not reduce postoperative nausea and vomiting in female patients.

The analgesic effect of transversus abdominis plane block at gynaecologic transabdominal surgery

2014, 34(4):  523-526. 

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Objective To assess the clinical analgesic effect of transversus abdominis plane (TAP) block at transabdominal hysterectomy and myomectomy gynaecological surgeries. Methods Forty patients (ASA I/II) undergoing transabdominal hysterectomy or myomectomy gynaecological surgery were randomly divided into control group ( n=20) and TAP block group (n =20). Ultrasound-guided TAP block was practiced after general anesthesia induction, ropivacaine and normal saline were injected in TAP block group and control group separately. Intra-operative fentanyl consumption, post-operative rescue analgesic requirements, and post-operative NRS pain score were compared between the two groups. Results Intro-operative fentanyl consumption and post-operative rescue analgesic demand rate of TAP group were less than those of control group（P<0.05）. At the time of 4 hours after surgery, NRS pain score of TAP group was less than that of control group（P<0.05）. Conclusions TAP block can provide safe intro- and post-operative analgesia for transabdominal hysterectomy and myomectomy gynaecological surgeries.

The value of T-SPOT.TB in the differential diagnosis of Sarcoidosis and Pulmonary Tuberculosis

2014, 34(4):  527-530. 

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Objective T-SPOT.TB is a new method to diagnose tuberculous infection through detecting the frequencies of IFN-γ producing T cells which respond to the M.tuberculosis specific antigens encoded by the RD1 region(ESAT-6 and CFP-10). This study aimed to evaluate the role of T-SPOT.TB in the differentiation of the sarcoidosis and tuberculosis. Methods The data of T-SPOT.TB of 44 sarcoidosis and 30 pulmonary tuberculosis patients in PUMCH between January 2008 and March 2011 were analysed. Results Among the 44 sarcoidosis patients ,the T-SPOT.TB result was positive in 12 patient (27.3%) ,while among the 30 pulmonary tuberculosis patients ,the T-SPOT.TB result was positive in 27 patient (90%) .Pooled frequencies of ESAT-6 and CFP-10 specific IFN-γ secreting T cell was 46SFCs/106PBMC（IQR 28～204） for sarcoidosis patients ,significantly lower than that for pulmonary tuberculosis patients which was 565 SFCs/106PBMC（IQR 170～2340）. Conclusion T-SPOT.TB may be a new method for the differentiation of sarcoidosis from pulmonary tuberculosis.

Mechanism involved in damp-heat induced mice myocardial cell apoptosis

2014, 34(4):  531-535. 

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Objective To explore the mechanism of damp-heat induced mice myocardial cell apoptosis. Method In this study, mice was exposed to damp-heat (42 ℃, RH 65%) (H group) environment or room temperature (C group). The myocardial cell apoptosis rates in tissues were analyzed by TUNEL staining. The expression levels of Ang II were detected by EIISA assay. Cardiomyocytes of rats with 1 day were pretreated with caspase-3 inhibitor Z-DEVD-FMK, or p38MAPK inhibitor SB203580, for 24 h, followed by culturing with the indicated dose of Ang II. AnnexinV-FITC was used to analyze cell apoptosis ratio. In addition, the expression of caspase-3 and p38MAPK was assessed by Western blotting. Results The rates of cell apoptosis and Ang II expression levels in H group were significantly higher than those in C group (P<0.05). In vitro, Ang II dose-dependently induced cardiomyocytes apoptosis, accompany with the up-regulation of caspase-3 and P38 MAPK expression. When preconditioning with Z-DEVD-FMK, the apoptotic ratio of cardiomyocytes was significantly attenuated in Ang II-treated group, implying that Ang II triggered cell apoptosis in caspase-3-depedent manner. Additionally, pretreatment with SB203580 dramatically abrogated caspase-3 expression induced by Ang II. Conclusion Damp-heat environment induced cardiomyocytes apoptosis by Ang II-activated p38MAPK-caspase-3 pathway. Consequently, Ang II may be a potential target for innovative strategies against cardiovascular diseases induced by Damp-heat environment.

The viability and the transplanted survival rate analysis of fat granule in cryopreservation with different frozen conditions

2014, 34(4):  536-540. 

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Objective To investigate whether the high glucose Dulbecco’s Modified Eagle Medium and fetal bovine serum as base fluid are benefit for improve the viability and post-transplant survival rate of frozen adipose tissue. Methods Fat granules was cryopreserved at -180℃ in physiological saline, high glucose Dulbecco’s Modified Eagle Medium and fetal bovine serum respectively, adding with 8%DMSO. Rewarming at 4 weeks, the viability and post transplantation survival rate of frozen fat were determined by trypan blue staining, creatine kinase activity assay, MTT assay and xenotransplantation experiments. Results Frozen adipose tissue in fetal bovine serum adding with 8%DMSO showed an increase in cell viability and improvement in post transplantation survival rate (p<0.01). Conclusion FBS as a base fluid can significantly improve the cell vitality and post transplantation survival rate of cryopreservation adipose tissue.

Clinical diagnosis and treatment of multilocular cystic nephroma

2014, 34(4):  541-543. 

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Objective To determine the diagnosis and treatment of multilocular cystic nephroma．Methods We conducted a retrospective analysis, aiming to provide diagnosis and therapy for multilocular cystic nephroma. 6 cases were involved in this analysis, including three males and three females aged between 29～51 years old． Among the 6 cases, 3 were asymptomatic, 2 present with backache and 1 with macroscopic hematuria. Course of disease were between 10 days to 3 years. All cases were unilateral, 3 located in the left kidney and 3 in the right. 4 located in the lower pole and 2 in the upper pole. 5 cases were diagnosed with cystic lesions, with septum inside and 2 cases with calcification.1 case were diagnosed with malignant lesion. 4 cases undergo CT and 3 cases undergo MRI, all indicated multilocular cystic lesions, 2 cases with linear and patchy enhancement in MRI. IVP of the one case with hematuria indicated damage of the calyces and overflow of contrast agents. Results Open surgery had been carried out in all of the 6 patients, including 5 partial nephrectomy and 1 nephrectomy．All the 6 patients had been cured of the disease with no recurrence during the 1 to 15 years’ of follow up．Conclusion Ultrasonic type B and CT scanning are the major means of diagnosis before surgery．Surgery is the main choice of treatment．Nephron-sparing resection of tumor is a primary and effective therapeutic method for this disease.

The Comparison of Two Nonviral Vectors mediated microRNA transfection

2014, 34(4):  544-545. 

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The effect of the Glut1 RNA interference on the proliferation of MCF-7 cells

2014, 34(4):  546-547. 

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Effects of resveratrol on the proliferation and expression of MsrA, CXCR4 and MMP-13 in colon cancer SW480 cells

2014, 34(4):  548-549. 

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Research progress in biological characteristics of Candida albicans

2014, 34(4):  550-554. 

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With the increased application of broad-spectrum antibiotics, immunosuppression, and cytotoxic therapies, Candida albicans, being an conditioned pathogen, has become one of the common nosocomial infection pathogens. Recent studies showed that the pathogenicity of Candida albicans depends upon hypothetical virulence factors including the adhesion to host cells, transition from yeast to mycelium, and production of secreted hydrolytic enzymes. The impacts on the evolution of drug resistance and adaptation to environmental stress rely on multiple machanisms and remains largely unexplored. Therefore, to understand the basic structure, genotype, pathogenesis, drug resistance of Candida albicans and the influence of environmental stress on Candida albicans has great clinical significance in disease diagnosis, treatment and prevention.

Progress on Discoidin Domain Receptor1

2014, 34(4):  555-558. 

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Discoidin domain receptor1(DDR1), a novel receptor tyrosine kinase(RTK), specifically binds to and is activated by collagen, consequently triggering a sequence of transmembrane signal transmission functions of cells. Many disease processes such as the development of tumor, inflammatory response and fibrosis have abnormal expressions of DDR1.

Autophagy in Liver Diseases

2014, 34(4):  559-561. 

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Autophagy is a process of bulk degradation of proteins and organelles in cytoplasm．Recent studies indicate that there is a close relationship between autophagy and liver diseases. It provides us a promising avenue for liver diseases therapy.

Advance in the study of adipokines in osteoarthritis

2014, 34(4):  562-565. 

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Recent studies demonstrated that adipokines, white adipose tissue produced endogenous bioactive peptides, may have play an critical role in pathophysiology of osteoarthritis(OA). It have been showed that adipokines, involving leptin, adiponectin, visfatin, and resistin, may have contributed not only to inflammation process of OA as proin?ammatory factors but to cartilage degeneration of OA late stage by metalloproteinases alone or in combination with in?ammatory mediators.

Clinical Application Value of gastric carcinoma relevant miRNA

2014, 34(4):  566-569. 

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Specific miRNA in gastric cancer tissue or peripheral blood plasma played an important role in the pathogenesis of gastric carcinoma including occurrence and development. Some specific miRNA expression level could prompt the occurrence and stages of gastric cancer, invasion and metastasis ability and whether metastasis has occurred, the possibility of postoperative recurrence and survival time of patients as well as whether the chemical drug treatment is effective.

Behavior making doctors prescribe antibiotics in acute upper respiratory tract infections

2014, 34(4):  570-572. 

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Objective This study aims to analyze doctor’s behavior of prescribing antibiotics in acute respiratory tract infections (AURTI). Methods 40 senior residents from Peking Union Medical College Hospital who have emergency medical training background were interviewed with designed questionnaire. Results When the doctors prescribe antibiotics in acute respiratory tract infections, they mainly rely on the result of complete blood count and symptoms like purulent discharge, fever and tonsil exudate. Most of the doctors haven’t received any training on AURTI or read any related papers or guideline. Conclusion Commonness exists within doctors, which highly rely on some specific symptoms, signs and test results. And the education of AURTI in doctors is insufficient.

Study on content and approaches of professional quality cultivation in eight-year program medical students of China

2014, 34(4):  573-576. 

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Despite the eight-year program medical students training objectives has no agreement, the concept of "elite education" has clinical hospital in the medical colleges and universities, and even the social consensus. Based on the definition of eight-year program medical students in our country, on the basis of professional quality concept, through the literature method, comparison analysis method, and analyzes the several eight-year program medical students training institutions in the professional quality cultivation ways and means, and on this basis, put forward China's eight-year program medical students professional quality raised some suggestions, so as to colleges and universities in the eight-year program medical students professional literacy cultivation work to provide the reference, to improve the eight-year program medical students professional quality to cultivate scientific research level.