Basic & Clinical Medicine ›› 2011, Vol. 31 ›› Issue (7): 762-766.
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Chang LIU1,Wei LIU2,Ming WANG2,Bin YIN3,Jian-gang YUAN1
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Abstract: Objective To investigate the role of miR-16 in cell cycle and apoptosis in Neuro2a cells and explore the possible function of miR-16 in tumor. Methods The overexpression plasmid pcDNA3.1-miR-16 of miR-16 was cloned and introduced into Neuro2a cells. Real-time PCR was chosen to test the overexpression of miR-16. Cell cytometry was used to study the role of miR-16 in cell cycle and apoptosis, and apoptosis cells were quantitatively examined by TUNEL. Results After tranfection of pcDNA3.1-miR-16, the mature miR-16 was significantly overexpressed (P<0.05). Cell cytometry results showed that the cell cycle didn’t change after miR-16 overexpression while cell apoptosis were found and confirmed by TUNEL. Conclusion There was obvious apoptosis of Neuro2a cell after overexpression of miR-16, thus, miR-16 might involve in cure of tumor.
Key words: miR-16, Neuro2a cells, cell cycle, apoptosis, tumor
CLC Number:
R739.4
Chang LIU Wei LIU Ming WANG Bin YIN Jian-gang YUAN. Overexrpession of miR-16 promotes apoptosis in Neuro2a cells[J]. Basic & Clinical Medicine, 2011, 31(7): 762-766.
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2011/V31/I7/762