Basic & Clinical Medicine ›› 2023, Vol. 43 ›› Issue (5): 751-754.doi: 10.16352/j.issn.1001-6325.2023.05.0751

• Original Articles • Previous Articles     Next Articles

Influence of PABPC1 on translation activity in human monocytic leukemia cell line THP-1

ZHAI Xueying, WANG Fang, YU Jia*   

  1. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, China
  • Received:2023-02-21 Revised:2023-03-22 Online:2023-05-05 Published:2023-04-26
  • Contact: *

Abstract: Objective To investigate the effect of cytoplasmic poly(A) binding protein-1(PABPC1) on translation of human monocytic leukemia cells line THP-1. Methods Specific short hairpin RNA(shRNA) was transfected into THP-1 cells. Western blot was used to verify the interference efficiency. The polysome profiling was used to examine the translation ability of THP-1 cells. Results The expression of PABPC1 protein was decreased in shPABPC1 transfected THP-1(P<0.05). Compared with control group, the translation ability of THP-1 cells in shPABPC1 group was reduced. Conclusions Knockdown of PABPC1 may inhibit the translation in THP-1 cells and PABPC1 is a potential target for the treatment of acute myeloid leukemia (AML).

Key words: acute myeloid leukemia (AML), cytoplasmic poly(A) binding protein-1, translation

CLC Number: