Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (7): 1071-1076.doi: 10.16352/j.issn.1001-6325.2022.07.1071

• Original Articles • Previous Articles     Next Articles

Lnc-SLC2A12-10:1 silencing inhibits the proliferation, migration and invasion of gastric cancer cell line AGS

LIU Lei1, LI Shi-bao2, ZHANG Hao-liang2*   

  1. 1. Department of Physiology, Xuzhou Medical University, Xuzhou 221004;
    2. Department of Laboratory Medicine, the Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China
  • Received:2021-03-25 Revised:2021-10-15 Online:2022-07-05 Published:2022-06-29
  • Contact: *

Abstract: Objective To investigate the effect of long non-coding RNA(lncRNA) lnc-SLC2A12-10:1 silencing on the proliferation, migration and invasion of gastric cancer cell line AGS. Methods Gene interference technology was used to construct the lnc-SLC2A12-10:1 silenced gastric cancer cell strain AGS, and RT-qPCR was used to verify the interference efficiency by lnc-SLC2A12-10:1 silencing. The proliferation of AGS cell was examined by CCK-8 method. The cell migration and invasion were evaluated by wound healing and Transwell assay. Bioinformatics prediction software was applied to predict lnc-SLC2A12-10:1 targeting miRNA and to verify them with RT-qPCR. Results Lnc-SLC2A12-10:1 shRNA effectively silenced expression of lnc-SLC2A12-10:1, and lnc-SLC2A12-10:1 silencing inhibited the proliferation of gastric cancer cells (P<0.05). Lnc-SLC2A12-10:1 silencing also inhibited the migration and invasion of AGS cells (P<0.05). It had been demonstrated that lnc-SLC2A12-10:1 targeting miRNAs might be hsa-miR-105-5p and hsa-miR-150-3p. Conclusions Lnc-SLC2A12-10:1 silencing can significantly inhibit the proliferation, migration and invasion of AGS cells.

Key words: long noncoding RNA, gastric cancer, proliferation, migration

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