Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (6): 871-875.doi: 10.16352/j.issn.1001-6325.2022.06.001

• Original Articles • Previous Articles     Next Articles

Peripheral blood miR-200c level predicts the occurence of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation

WU Kun1, NIE Bo2, LI Yun-tao2, YANG Jin-rong2, CHENG Shen-ju1, LI Yan-hong1, SHI Ming-xia2*   

  1. 1. Department of Laboratory Medicine; 2. Department of Hematology, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
  • Received:2021-01-11 Revised:2021-09-03 Online:2022-06-05 Published:2022-06-02
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Abstract: Objective To investigate the predictive function of miR-200c level in peripheral blood on acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation. Methods Fifty-one patients who received allo-HSCT in hematology department of our hospital from January 2014 to October 2018 were selected as the research subjects. According to the principle of whether agVHD occurred after transplantation, 33 patients were in the experimental group (with aGVHD) and 18 patients were in the control group (without aGVHD). RT-qPCR was used to detect miR-200c in two groups and the differences in miR-200c expression between the two groups were analyzed. Results The transplantation was successful in both groups, and the hematopoietic function was restored. The recovery time was 18 days, among which 33 patients developed aGVHD and 18 patients did not develop aGVHD, with an incidence of 64.71%. The expression of miR-200c in experimental group was lower than that in control group before transplantation and 7, 14, and 72 d after transplantation (P<0.05). MiR-200c in peripheral blood after the occurrence of aGVHD was significantly lower than that before the occurrence(P<0.001). Compared with aGVHD Ⅱ, the expression of miR-200c in peripheral blood of aGVHD Ⅱ patients was down-regulated (P<0.05); as compared with aGVHD Ⅲ patients, the expression of miR-200c in peripheral blood of aGVHD Ⅲ patients was down-regulated (P<0.001); the 2-year survival rate in the high expression group of miR-200c was higher than that in the low expression group (P<0.05). Conclusions The reduced level of miR-200c is associated with the occurrence of aGVHD after allogeneic hematopoietic stem cell transplantation. With the increase of the severity of aGVHD the expression level of miR-200c is decreased, and the survival rate of patients with low miR-200c expression is low, so the expression of miR-200 is a potential marker for early diagnosis and prediction of aGVHD.

Key words: acute graft-versus-host disease, miR-200c, allogeneic hematopoietic stem cell transplantation, prognosis

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