Basic & Clinical Medicine ›› 2021, Vol. 41 ›› Issue (9): 1277-1284.

• Original Articles • Previous Articles     Next Articles

Circ_0038467 targets at miR-182 for regulating S. pneumoniae (Sp)-induced injury of human alveolar epithelial cell line HPAEpiC

LIU Qing1, ZHANG Lei2, ZHANG Li-yuan3, WANG Shi-feng4, ZUO Li-min1*   

  1. 1. Department of Emergency; 2. Department of Respiratory Medicine; 3. Clinical Laboratory; 4. Department of Pathology, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Techology of China, Chengdu 611731, China
  • Received:2020-09-22 Revised:2021-02-02 Online:2021-09-05 Published:2021-09-02
  • Contact: *fob6er@163.com

Abstract: Objective To investigate the effect of circular RNA 0038467 (circ_0038467) targeting at miR-182 on apoptosis and endoplasmic reticulum stress of alveolar epithelial cell line(HPAEpiC) induced by S. pneumoniae (Sp). Methods HPAEpiC cells were divided into control (NC) group (without any treatment), Sp group (Sp infection), Sp+si-NC group (Sp infection after transfection by si-NC), Sp+si-circ_0038467 group (Sp infection after transfection by si-circ_0038467), Sp+miR-NC group (Sp infection after transfection of miR-NC), and Sp+miR-182 group (Sp infection after transfection of miR-182 mimics), Sp+si-circ_0038467+anti-miR-NC group (Sp infection after transfection by si-circ_0038467 and anti-miR-NC), Sp+si-circ_0038467+anti-miR-182 group (Sp infection after transfection by si-circ_0038467 and anti-miR-182). The expressions of circ_0038467 and miR-182 were detected by RT-qPCR. Flow cytometry detected cell apoptosis; Western blot analyzed the expression level of B-cell lymphoma (Bcl-2), heavy chain binding protein (BIP), Bcl associated X protein (Bax) and CCAAT enhancer binding protein homologous protein (CHOP), cysteine protease 3 (caspase-3), and cleaved-caspase-3 proteins. The effects of circ_0038467 and miR-182 expression on HPAEpiC cell apoptosis and endoplasmic reticulum stress induced by S. pneumoniae were analyzed. Results The apoptosis rate of HPAEpiC cells, the expression of circ_0038467, Bax, caspase-3, cleaved-caspase-3, BIP and CHOP were significantly increased after Sp infection (P<0.05), whereas the expression of Bcl-2 was significantly decreased(P<0.05). After circ_0038467 inhibition or miR-182 over-expression, the apoptosis rate, expression of circ_0038467, Bax, caspase-3, cleaved-caspase-3, BIP and CHOP were significantly reduced (P<0.05), whereas Bcl-2 expression was significantly increased(P<0.05). miR-182 inhibition can attenuate the effect of circ_0038467 inhibition on Sp induced HPAEpiC apoptosis and endoplasmic reticulum stress (P<0.05). Conclusions Inhibition of circ_0038467 can attenuate Sp induced alveolar epithelial cell apoptosis and endoplasmic reticulum stress, and its mechanism is related to the targeted regulation of miR-182 expression.

Key words: circ_0038467, S. pneumoniae, alveolar epithelial cells, apoptosis, endoplasmic reticulum stress

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