Histone acetylation is decreased in X-ray radiation-induced injury of rat myocardial cell line H9c2

Abstract

Abstract: Objective To investigate the effects of X-ray radiation on H9c2 cells and histone acetylation patterns, and to analyze the correlation between them. Methods H9c2 cells were randomly divided into control group and different model groups. The cells of model groups were irradiated with different doses of 2,4,6,8 Gy of X-ray radiation respectively. The activity of LDH in supernatants, the activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) in cells were detected by chromatometry. Cell apoptotic ratio was measured by annexin V-FITC/PI staining and flow cytometry analysis. Apoptosis-related protein Bcl-2,caspase-3 and histone acetylation related protein H3K9ac,HDAC3 were detected by Western blot. Results Compared with control group, the relative activity of LDH in supernatants and relative content of malondialdehyde (MDA) in H9c2 cells were increased (P＜0.05) in model groups, the relative activity of intracellular superoxide dismutase (SOD) was decreased(P＜0.05) in model groups. The apoptotic rate of H9c2 cells was increased in model groups compared with control group(P＜0.05). The protein expression of caspase-3 increased (P＜0.05), the expression of Bcl-2 decreased in model groups(P＜0.05), and also found that the protein expression of H3K9ac significantly decreased and the expression of HDAC3 significantly increased in model groups(P＜0.05). Conclusions X-ray radiation can induce H9c2 cells' injury and apoptosis,accompanied by the decrease of intracellular histone acetylation level, this change may be related to the increase of HDAC3 expression.

Key words: histone acetylation, radiation, cardiomyocyte

CLC Number:

R541.9

MENG Ying, ZHANG Zheng-yi, ZHAO Yuan, LU Chang-hong, SHANG Bo, SUN Peng-fei, ZHANG De-kui. Histone acetylation is decreased in X-ray radiation-induced injury of rat myocardial cell line H9c2[J]. Basic & Clinical Medicine, 2021, 41(8): 1139-1143.

References

[1]Ratosa I, Ivanetic Pantar M. Cardiotoxicity of mediastinal radiotherapy[J]. Rep Pract Oncol Radiother, 2019, 24: 629-643.
[2]Gernaat SAM, Ho PJ, Rijnberg N, et al. Risk of death from cardiovascular disease following breast cancer: a systematic review[J].Breast Cancer Res Treat, 2017, 164: 537-555.
[3]Cheng YJ, Nie XY, Ji CC, et al. Long-term cardiova-scular risk after radiotherapy in women with breast cancer[J]. Am Heart Assoc, 2017, 6: e005633. doi: 10.1161/JAHA.117.005633.
[4]Slezak J, Kura B, Babal P, et al. Potential markers and metabolic processes involved in the mechanism of radiation-induced heart injury[J].Can J Physiol Pharmacol, 2017, 95: 1190-1203.
[5]Koprinarova M, Schnekenburger M, Diederich M. Role of histone acetylation in cell cycle regulation[J]. Curr Top Med Chem, 2016, 16: 732-744.
[6]Li YM, Du W, Zhao RP, et al. New insights into epigenetic modifications in heart failure[J]. Front Biosci (Landmark Ed), 2017, 22:230-247.
[7]Wang YG, Miao X, Liu YC, et al. Dysregulation of histone acetyltransferases and deacetylases in cardiova-scular disease[J]. Oxid Med Cell Longev, 2014: 641979.doi: 10.1155/ 2014/ 641979.
[8]Bakshi MV, Barjaktarovic Z, Azimzadeh O, et al. Long-term effects of acute low-dose ionizing radiation on the neonatal mouse heart: a proteomic study[J]. Radiat Environ Biophys, 2013, 52: 451-461.
[9]Yamamori T, Yasui H, Yamazumi M, et al. Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint[J]. Free Radic Biol Med, 2012, 53: 260-270.
[10]Antwih DA, Gabbara KM, Lancaster WD, et al. Radiation-induced epigenetic DNA methylation modification of radiation-response pathways[J]. Epigenetics, 2013, 8: 839-848.
[11]Li XH, Ha CT, Fu DD, et al. Delta-tocotrienol suppres-ses radiation-induced microRNA-30 and protects mice and human CD34⁺ cells from radiation injury[J]. PLoS One, 2015, 10: e0122258. doi: 10.1371/ journal. pone. 0122258.
[12]Zhang XR, Kluz T, Gesumaria L, et al. Solar simulated ultraviolet radiation induces global histone hypoacetylation in human keratinocytes[J]. PLoS One, 2016, 11: e0150175. doi: 10.1371/journal.pone.0150175.
[13]Yang XJ, Seto E. HATs and HDACs: From structure, function and regulation to novel strategies for therapy and prevention[J]. Oncogene, 2007, 26: 5310-5318.
[14]Zhu H, Shan L, Schiller PW, et al. Histone deacetylase-3 activation promotes tumor necrosis factor-alpha (TNF-alpha) expression in cardiomyocytes during lipopolysaccharide stimulation[J]. J Biol Chem, 2010, 285: 9429-9436.

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