Correlation of serum miR-181b and miR-27a expression with the severity of carotid atherosclerosis

Abstract

Abstract: Objective To identify the relationship between expression of serum microRNA (miR)-181b, miR-27a and the severity of carotid atherosclerosis (CAS). Methods Totally 102 patients with dyslipidemia were selected and 100 healthy people as controls.RT-qPCR was used to detect the expression of serum miR-181b and miR-27a. The expression of miR-181b and miR-27a of patients with dyslipidemia was compared with that of the control group. The influencing factors of the occurrence of CAS in patients with dyslipidemia and the correlation between serum miR-181b, miR-27a levels and the degree of CAS lesions were investigated. Results The relative expression of miR-181b in dyslipidemia patients was lower than that of the control group, while the relative expression of miR-27a was higher than that of the control group (P＜0.05). The expression of miR-181b in the cirrhosis group was lower than that in the non-cirrhotic group, and the relative expression of miR-27a, hs-CRP, LDL-C levels, and the proportion of hypertension were higher than those in the non-cirrhotic group (P＜0.05). Logistic regression analysis showed that the above indicators were independent factors influencing the occurrence of CAS in patients with dyslipidemia(P＜0.05). ROC showed that the sensitivity of relative expression of miR-181b and miR-27a in serum was 84.75% and 81.36% in the predicting the occurrence of CAS in patients with dyslipidemia, the specificity was 76.74% and 74.42%, and the accuracy was 81.37% and 78.43%, respectively, AUC was 0.791, 0.750 respectively. Pearson correlation analysis showed that the relative expression of serum miR-181b was negatively correlated with Crouse score(P＜0.05), and the relative expression of miR-27a was positively correlated with Crouse score(P＜ 0.05). Conclusions The expression of miR-181b and miR-27a in serum is abnormal in patients with dyslipidemia, and both of them are independent influencing factors of CAS, and their expression level is positively related to the severity of the disease.

Key words: carotid atherosclerosis, dyslipidemia, miR-181b, miR-27a

CLC Number:

R543.4

ZHANG Chuan-xi, ZHANG Yu, GUO Sha-sha, LI Jing, YUAN Yi-qiang, WANG Ming-jie. Correlation of serum miR-181b and miR-27a expression with the severity of carotid atherosclerosis[J]. Basic & Clinical Medicine, 2021, 41(8): 1133-1138.

References

[1]Lioudaki S, Verikokos C, Kouraklis G, et al. Paraoxonase-1: characteristics and role in atherosclerosis and carotid artery disease[J]. Curr Vasc Pharmacol, 2019, 17:141-146.
[2]Yahagi K, Kolodgie FD, Lutter C, et al. Pathology of human coronary and carotid artery atherosclerosis and vascular calcification in diabetes mellitus[J]. Arterioscler Thromb Vasc Biol, 2017, 37:191-204.
[3]Maitrias P, Metzinger-Le Meuth V, Nader J, et al. The involvement of miRNA in Carotid-Related Stroke[J]. Arterioscler Thromb Vasc Biol, 2017, 37:1608-1617.
[4]Magenta A, Sileno S, D'Agostino M, et al. Atherosc-lerotic plaque instability in carotid arteries: miR-200c as a promising biomarker[J]. Clin Sci (Lond), 2018, 132:2423-2436.
[5]Wu Y, Zhang F, Lu R, et al. Functional lncRNA-miRNA-mRNA networks in rabbit carotid atherosclerosis[J]. Aging (Albany NY), 2020, 12:2798-2813.
[6]杨颖, 霍勇. 颈动脉粥样硬化超声检测方法的规范化——“冠心病早期诊断和综合治疗技术体系的研究”颈动脉超声检测方案[J]. 中国介入心脏病学杂志, 2008, 16:42-43.
[7]诸骏仁, 高润霖, 赵水平, 等. 中国成人血脂异常防治指南(2016年修订版)[J]. 中国循环杂志, 2016, 16:15-35.
[8]徐树东, 贺江波. 冠心病患者AASI、IMT、Crouse积分及血清选择素水平的变化及意义[J]. 检验医学与临床, 2018, 15:1838-1841.
[9]Su Q, Ye Z, Sun Y, et al. Relationship between circulating miRNA-30e and no-reflow phenomenon in STEMI patients undergoing primary coronary intervention[J]. Scand J Clin Lab Invest, 2018, 78:318-324.
[10]Looney AM, O'Sullivan MP, Ahearne CE, et al. Altered expression of umbilical cord blood levels of miR-181b and its downstream target mUCH-L1 in infants with moderate and severe neonatal hypoxic-ischaemic encephalopathy[J]. Mol Neurobiol, 2019, 56:3657-3663.
[11]Xing Y, Li J, Li SP, et al. miR-27a-5p regulates apoptosis of liver ischemia-reperfusion injury in mice by targeting Bach1[J]. J Cell Biochem, 2018, 119:10376-10383.
[12]Liu JY, Shang J, Mu XD, et al. Protective effect of down-regulated microRNA-27a mediating high thoracic epidural block on myocardial ischemia-reperfusion injury in mice through regulating ABCA1 and NF-κB signaling pathway[J]. Biomed Pharmacother, 2019, 112:108606. doi:10.1016/j.biopha.2019.108606.
[13]Wang L, Qi Y, Wang Y, et al. LncRNA MALAT1 Suppression protects endothelium against oxLDL-induced inflammation via inhibiting expression of miR-181b target gene TOX[J]. Oxid Med Cell Longev, 2019:8245810.doi:10.1155/2019/8245810.
[14]朱磊, 路瑛丽, 冯连世. 低氧训练诱导miR-27/PPARγ调控肥胖大鼠肝脏脂肪酸代谢变化的研究[J]. 中国体育科技, 2018, 54:115-122.