Up-regulation of miR-433 expression inhibits proliferation of colorectal cancer cell line in vitro

Abstract

Abstract: Objective To study the expression and function of miR-433 in the development of colorectal cancer. Methods RT-qPCR was used to detect the expression of miR-433 in clinical specimens and serum of patients with colorectal cancer, and compared with those from adjacent tissues or at different stages of tumore development and from normal persons. The colorectal cancer cell lines HT-29, HCT-116, SW480 and normal colon cell line NCM460 were cultured, and the expression of miR-433 was measured by real-time quantitative PCR. Subsequently, miR-433 mimic was transfected into SW480 cells,CCK-8 was used to detect cell proliferation, flow cytometry was used to detect cell cycle changes, scratch test and Transwell test were used to detect cell migration and invasion respectively. Results Compared with the adjacent tissues, the expression of miR-433 in colorectal cancer tissues was significantly decreased(P＜0.05). The serum level of miR-433 in patients with colon cancer was also significantly lower than that in normal persons(P＜0.05). In addition, from stage Ⅰ to stage Ⅳ of TNM, the expression of miR-433 in colorectal cancer tissues gradually and significantly decreased. Compared with normal human colon epithelial cell line NCM460, the expression of miR-433 was down-regulated in colorectal cancer cell lines HT-29, HCT-116 and SW480(P＜0.05). Compared with the control group, the proliferation of SW480 cells was significantly inhibited(P＜0.05) and the proportion of G2 and M phase cells increased after SW480 transfection. The invasion and migration of cells decreased significantly after up-regulation of miR-433(P＜0.05). Conclusions The expression of miR-433 is decreased in colorectal cancer. Up-regulation of its expression in SW480 cells inhibits cell proliferation, invasion and migration.

Key words: colorectal cancer, proliferation, miR-433

CLC Number:

R735

ZHANG Jian, ZHANG Tong, WANG Hong-wei, ZHANG Ji-hong, ZHANG Bao-zhen, DONG Xin-min. Up-regulation of miR-433 expression inhibits proliferation of colorectal cancer cell line in vitro[J]. Basic & Clinical Medicine, 2021, 41(8): 1127-1132.

References

[1]Bleijenberg A, IJspeert J, Carballal S, et al. Low incidence of advanced neoplasia in serrated polyposis syndrome after (sub)total colectomy: results of a 5-year international prospective cohort study[J]. Am J Gastroenterol,2019,114:1512-1519.
[2]Ladabaum U, Dominitz JA, Kahi C,et al. Strategies for colorectal cancer screening[J]. Gastroenterology,2020,158:418-432.
[3]Bobińska K, Szemraj J, Mossakowska-Wójcik J, et al. The significance of microRNAs in the course of rDD[J]. Pharmacol Rep, 2017,69:206-212.
[4]Huang M, Xie X, Song X, et al. miR-506 suppresses colorectal cancer development by inhibiting orphan nuclear receptor NR4A1 expression[J].Cancer,2019, 10: 3560-3570.
[5]Hindle AG, Thoonen R, Jasien JV, et al. Identification of candidate miRNA biomarkers for glaucoma invest ophthalmol[J]. Vis Sci, 2019, 60: 134-146.
[6]Yan L, You WQ, Sheng NQ, et al. A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer[J]. Aging (Albany NY),2018,10: 3774-3793.
[7] Lee SC, Quinn A, Nguyen T, et al. A cross-cancer metastasis signature in the microRNA-mRNA axis of paired tissue samples[J].Mol Biol Rep,2019,46:5919-5930.
[8]Bai X, Lu D, Lin Y, et al. A seven-miRNA expression-based prognostic signature and its corresponding potential competing endogenous RNA network in early pancreatic cancer[J]. Exp Ther Med,2019,18: 1601-1608.
[9]Ursu S, Majid S, Garger C, et al. Novel tumor suppressor role of miRNA-876 in cholangiocarcinoma[J].Oncogenesis,2019,8: 42.doi:10.1038/s41389-019-0153-z.
[10]Zhang T, Jiang K, Zhu X, et al. miR-433 inhibits breast cancer cell growth via the MAPK signaling pathway by targeting Rap1a[J]. Int J Biol Sci, 2018, 14: 622-632.
[11]Tarale P, Daiwile AP, Sivanesan S, et al. Manganese exposure: linking down-regulation of miRNA-7 and miRNA-433 with α-synuclein overexpression and risk of idiopathic Parkinson's disease[J]. Toxicol Vitro, 2018,46:94-101.
[12]Li H, Li J, Yang T, et al. microRNA-433 represses proliferation and invasion of colon cancer cells by targeting homeobox A1[J]. Oncol Res, 2018,26:315-322.

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