Abstract: Objective To investigate the expression of perilipin 2 in gastric cancer and its effect on the proliferation, invasion and migration of cancer cells. Methods Human normal gastric mucosa cell line GES-1 and human gastric cancer cell line NCI-N87 were selected. NCI-N87 cells were divided into three groups. control group received no treatment, NC-shRNA group was transfected with 10 μg NC-shRNA recombinant lentivirus plasmid, and perilipin2-shRNA group was transfected with 10 μg perilipin2-shRNA recombinant lentivirus plasmid. The proliferation rate, invasion ability and migration ability of NCI-N87 cells in each group were detected by CCK8 method, Transwell chamber and scratch test, respectively. Western blot was used to detect the expression levels of perilipin2, p62,kelch-like ECH-associated protein 1(keap1), nuclear factor E2-related factor-2(Nrf2) pathway related proteins in NCI-N87 cells in each group. Results The expression level of perilipn2 protein in NCI-N87 cells was significantly higher than that in GES-1 cells(P＜0.05). After treatment for 6,12,24,36 and 48 h, proliferation rate of NCI-N87 cells in perilipin2-shRNA group was lower than that in control and NC-shRNA group(P＜0.05). The number of NCI-N87 cells crossing membrane in perilipin2-shRNA group was less than that in control and NC-shRNA group(P＜0.05). The scratch space in perilipin2-shRNA group was wider than that in control and NC-shRNA group(P＜0.05). The protein expression of perilipn2, p62, keap1 and Nrf2 in NCI-N87 cells of perilipin2-shRNA group was lower than that of control and NC-shRNA group (P＜0.05).Conclusions Perilipin2 is highly expressed in gastric cancer cells, and knockdown of perilipin2 expression can inhibit the proliferation, invasion and migration of gastric cancer cells, and its mechanism may be related to the obstruction of the p62-keap1-Nrf2 pathway.

Key words: perilipin2, gastric cancer, tumor biological behavior, p62-keap1-Nrf2 pathway