Basic & Clinical Medicine ›› 2021, Vol. 41 ›› Issue (4): 539-544.

• Original Articles • Previous Articles     Next Articles

Hypoxia microenvironment promotes invasion and proliferation of human breast cancer cell line MCF-7

WANG Hai-yan, HUANG Ming-quan, PAN Guang-rui, WANG Gui-lin, LIANG Bin, QUAN Yi*   

  1. Department of Breast Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
  • Received:2020-04-24 Revised:2020-07-31 Online:2021-04-05 Published:2021-04-05
  • Contact: *yancheng1118@163.com

Abstract: Objective To investigate the expression of HIF-1 α, PAR-1 and VEGF in breast cancer tissues from patients with type 2 diabetes and the effect of hypoxia microenvironment on the invasion and proliferation of human breast cancer cell line (MCF-7). Methods Thirty-seven specimens of breast cancer from patients with complicated with type 2 diabetes mellitus were examined by surgical resection. The expression of HIF-1α, PAR-1, VEGF and microvessel density (MVD) in breast cancer and paracancerous tissues were examined by immunohistochemistry. MCF-7 cells were divided into control group (5.6 mmol/L glucose), high glucose group (30 mmol/L glucose), hypoxia group (1% O2 5% CO2 94% N2) and high glucose plus hypoxia group. The cell morphology was observed by microscoped, the cell proliferation was detected by CCK-8 method, the invasive ability of cells was detected by Transwell chamber test, and the expression of HIF-1α, PAR-1, VEGF mRNA and protein were detected by RT-qPCR and Western blot. Results The expression of HIF-1α, PAR-1, VEGF and MVD count in breast cancer tissues were higher than those in periaracancerous tissues(P<0.05); the number of cells passing through the basement membrane and the cell survival rate of MCF-7 in the high glucose hypoxia group were significantly higher than those in the control group (P<0.01); and the expressions of HIF-1α, PAR-1, VEGF mRNA and protein in the hypoxia group and hypoxia high glucose group were significantly higher than those in the control group(P<0.05). Conclusions Hypoxia and high glucose can promote the proliferation and invasion and the expression of HIF-1α, PAR-1, VEGF of MCF-7 cells, which is a potential target for clinical detection and treatment of breast cancer of patients with type 2 diabetes.

Key words: breast cancer, hypoxia inducible factor 1α, vascular endothelial growth factor, tumor microenvironment

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