miR-30a inhibits autophagy of rat cardiomyocyte line H9c2 induced by angiotensin 2

Abstract

Abstract: Objective To explore the effect of miR-30a on autophagy of rat cardiomyocytes induced by angiotensin 2(Ang2). Methods 1)H9c2 cells were treated with Ang2 2×10^－6 mol/L and NE 5×10^－6 mol/L respectively for 48 h,miR-30a expression was measured by RT-qPCR, LC3 and Beclin-1 protein expression were detected by Western blot. 2)miR-30a mimics, miR-30a inhibitor and miR-30a NC were transfected into H9c2 cells by Lipofectamine2000 and incubated for 24 h, the transfection efficiency was detected by fluorescence microscope.RT-qPCR and Western blot were applied to detect the effect of miR-30a on autophagy of H9c2 induced by angiotensin 2. Results 1)Comparing with the control group, the expression of miR-30a mRNA in Ang2 treatment group and NE group was significantly decreased (P＜0.05). Protein expression of LC3 and Beclin-1 in Ang2 group and NE group was significantly increased (P＜0.001). 2)Comparing with Ang2 treatment group, the expression of LC3 and Beclin-1 protein in Ang2+ miR-30a mimics group significantly decreased (P＜0.001). Conclusions Ang2 reduces the expression of miR-30a and induces H9c2 cardiomyocyte autophagy, miR-30a mimics can inhibit Ang2 induced H9c2 autophagy.

Key words: autophagy, angiotensin 2 (Ang2), miR-30a

CLC Number:

YU Tian-le, MA Han, WU Xin-hua, CHEN Zhang-rong, SUN Biao, PENG Yuan, LIU Hong. miR-30a inhibits autophagy of rat cardiomyocyte line H9c2 induced by angiotensin 2[J]. Basic & Clinical Medicine, 2021, 41(4): 490-494.

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