Differential protein profiling in brain and spinal arteriovenous malformations

Abstract

Abstract: Objective To explore the differences of protein profiling between brain arteriovenous malformation (bAVM) and spinal AVM (sAVM). Methods Proteins were extracted from bAVM,sAVM,control cerebrovascular and control spinal vessel specimens. The protein level in each group was measured by TMT-labeled quantitative proteomics. Results Totally 3 102 protein samples from bAVM, sAVM,control cerebrovascular and control spinal vessel were examined by high-throughput quantitative proteomics. Among them the expression level of 852 proteins was dramatically changed in bAVM and 205 proteins in sAVM. Bioinformatic analysis showed that 304 proteins were highly expressed only in bAVM, most of them were mitochondria proteins, mainly enriched in electron transport chain and calcium regulation pathways, 97 proteins were specifically highly expressed in sAVM associated with extracellular matrix organization. Conclusions The mitochondrial dysfunction and calcium regulation imbalance may play important roles in the process of bAVM. Disturbed extracellular matrix organization and glycoproteins are potentially involved in sAVM progression.

Key words: brain arteriovenous malformation, spinal arteriovenous malformation, proteomics, mitochondria, extracellular matrix

CLC Number:

R363

WANG Nai-li, MENG Shu, QIU Wen-ying, WANG Xia. Differential protein profiling in brain and spinal arteriovenous malformations[J]. Basic & Clinical Medicine, 2021, 41(11): 1570-1576.

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