Predictive research of 5-hydroxytryptamine in severity of severe community-acquired pneumonia

Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (9): 1195-1200.

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Predictive research of 5-hydroxytryptamine in severity of severe community-acquired pneumonia

HE Quan¹, LIU Dan-yu^2,3, YE Jun¹, ZHANG Yan-li¹, ZHU Hua-dong², JIANG Cheng-yu^1*, XU Jun^2*

1. Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;
2. Department of Emergency, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730;
3. Department of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing 100034, China

Received:2020-07-12 Revised:2020-07-24 Online:2020-09-05 Published:2020-09-04
Contact: ^*jiang@pumc.edu.cn; xujunfree@126.com

Abstract

Abstract: Objective To search for potential prognostic markers for severe community-acquired pneumonia (SCAP) using non-targeted metabolomics. Methods Testing plasma samples from 30 healthy people and 68 SCAP patients (all collected from January 2015 to June 2019 at Peking Union Medical College Hospital) by liquid chromatography mass spectrometry (LC-MS). According to clinical prognosis, patients were divided into survive group (n=49) and non-survive group (n=19). OPLS-DA (multivariate statistical analysis) and Kruskal Wallis Test (single variable statistical analysis) were used to identify differential metabolites. Correlation analysis of clinical indicators and prognostic analysis were also used for differential metabolites. Results Totally 3 463 metabolites were detected in healthy people and SCAP patients by LC-MS. Among them, 126 metabolites were screened out by OPLS-DA andKruskal Wallis Test analysis methods. Prognostic analysis showed that the quantity of 5-hydroxytryptamine(5-HT) was significantly different between survive group and non-survive group (P＜0.05, AUC＞0.75). Correlation analysis of clinical indicators showed that 5-HT was positively correlated with oxygenation index (OI) and negatively correlated with acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score. Conclusions The 5-HT as a potential prognostic marker for SCAP, may predict the prognosis of SCAP patients.

Key words: 5-hydroxytryptamine(5-HT), severe community-acquired pneumonia(SCAP), prognostic markers, metabolomics

CLC Number:

R181.3+8

HE Quan, LIU Dan-yu, YE Jun, ZHANG Yan-li, ZHU Hua-dong, JIANG Cheng-yu, XU Jun. Predictive research of 5-hydroxytryptamine in severity of severe community-acquired pneumonia[J]. Basic & Clinical Medicine, 2020, 40(9): 1195-1200.

References

[1] Leoni D, Rello J. Severe community-acquired pneumonia: optimal management[J]. Curr Opin Infect Dis, 2017, 30:240-247.
[2] Lanks CW, Musani AI, Hsia DW. Community-acquired pneumonia and hospital-acquired pneumonia[J]. Med Clin North Am, 2019, 103:487-501.
[3] Singanayagam A, Chalmers JD, Hill AT. Severity assessment in community-acquired pneumonia: a review[J]. QJM, 2009, 102: 379-388.
[4] Ewig S, Birkner N, Strauss R, et al. New perspectives on community-acquired pneumonia in 388406 patients. Results from a nationwide mandatory performance measurement programme in healthcare quality[J]. Thorax, 2009, 64: 1062-1069.
[5] Kolditz M, Ewig S, Höffken G. Management-based risk prediction in community-acquired pneumonia by scores and biomarkers[J]. Eur Respir J, 2013, 41:974-984.
[6] Zhang A, Sun H, Wang X. Saliva metabolomics opens door to biomarker discovery, disease diagnosis, and treatment[J]. Appl Biochem Biotechnol, 2012, 168:1718-1727.
[7] Nicholson JK, Lindon JC. Systems biology: Metabonomics[J]. Nature, 2008, 455:1054-1056.
[8] Gershon MD. Serotonin: its role and receptors in enteric neurotransmission[J]. Adv Exp Med Biol, 1991, 294:221-230.
[9] Andrews PW, Bharwani A, Lee KR, et al. Is serotonin an upper or a downer? The evolution of the serotonergic system and its role in depression and the antidepressant response[J]. Neurosci Biobehav Rev, 2015, 51:164-188.
[10] Gershon MD, Tack J. The serotonin signaling system: from basic understanding to drug development for func-tional GI disorders[J]. Gastroenterology, 2007, 132:397-414.
[11] Margolis KG, Gershon MD. Enteric neuronal regulation of intestinal Inflammation[J]. Trends Neurosci, 2016, 39: 614-624.
[12] Miller MJ. Tissue oxygenation in clinical medicine: an historical review[J]. Anesth Analg, 1982, 61:527-535.
[13] Krafft P, Steltzer H, Hiesmayr M, et al. Mixed venous oxygen saturation in critically ill septic shock patients. The role of defined events[J]. Chest, 1993, 103:900-906.
[14] Liu W, Peng L, Hua S. Clinical significance of dynamic monitoring of blood lactic acid, oxygenation index and C-reactive protein levels in patients with severe pneumonia[J]. Exp Ther Med, 2015, 10:1824-1828.

Predictive research of 5-hydroxytryptamine in severity of severe community-acquired pneumonia

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