High density lipoprotein improves the symptoms of collagen-induced arthritis in mice

Abstract

Abstract: Objective To investigate the effects of high-density lipoprotein (HDL) on collagen-induced arthritis (CIA) animal model. Methods DBA/1 mice were divided into three groups: control group, CIA group and CIA+HDL group. Mice in the CIA and CIA+HDL groups were immunized with an emulsion of Freund's complete adjuvant and bovine type Ⅱ collagen on day 0. The control group was injected with saline. On day 21, the CIA group and CIA+HDL group were injected with an emulsion of Freund's incomplete adjuvant and bovine type Ⅱ collagen to streng then the immune response. The control group was injected with saline only. The CIA+HDL group was injected with HDL(10 mg/kg) on day 40 followed by HDL injection five times every 3 days. The control group and the CIA group were injected with the same volume of saline. Changes in mouse body weight and clinical scores ofarthritis were recor- ded.The mice were sacrificed on day 75, and the serum levels of tumor necrosis factor-α, anti-collagen-Ⅱ antibody IgG2a of the mice were measured using ELISA kit. Then the tissue sections of ankle joints were stained with hematoxylin-eosin to analyze the severity of rheumatoid arthritis in mice to evaluate the efficacy of high-density lipoprotein in the treatment of rheumatoid arthritis. Results Compared with the CIA group, intravenous injection of HDL at a dose of 10 mg/kg significantly alleviated the symptoms of rheumatoid arthritis in mice. The results showed that HDL alleviated the clinical symptom of arthritis and decreased the level of serum pro-inflammatory factor TNF-α and specific antibody of IgG2α (P＜0.05). Histopathological examination showed that HDL can smoothen the ankle articular surface, significantly decrease synovial hyperplasia, inflammatory cell and bone erosion and other pathological changes. Conclusions HDL can significantly alleviated the symptoms of collagen-induced rheumatoid arthritis of mice.

Key words: high-density lipoprotein, rheumatoid arthritis, tumor necrosis factor-α, pathological analysis

CLC Number:

R392

WANG Xin, DONG Qing-zhe, ZHANG Juan, LI Dong, AI Di, WANG Yun-long. High density lipoprotein improves the symptoms of collagen-induced arthritis in mice[J]. Basic & Clinical Medicine, 2020, 40(7): 960-964.

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