Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (7): 955-959.

• Original Articles • Previous Articles     Next Articles

DihydrotanshinoneⅠ promotes apoptosis of human esophageal squamous cell carcinoma cell line EC9706

WANG Xiu-mei, WANG Kang-kang, CHEN Xiao-yan, LUO Jin-jian*   

  1. Department of Gastroenterology, Zhengzhou Central Hospital, Zhengzhou University, Zhengzhou 450007, China
  • Received:2019-11-21 Revised:2020-03-24 Online:2020-07-05 Published:2020-06-29
  • Contact: *f123my@163.com

Abstract: Objective To investigate whether dihydrotanshinone Ⅰ could regulate the biological activities of proliferation, migration and apoptosis of esophageal squamous cell carcinoma cell line EC9706 through Wnt/β-catenin signaling pathway. Methods EC9706 cells were cultured in medium containing 0, 10, and 30 μmol/L dihydrotanshinoneⅠ solution, and cell proliferation was examined with a cell counting kit-8, cell migration was checked with scratch test. Western blot was used to detect β-catenin, cyclin D1 and c-myc protein expression in cells. Apoptosis was detected by flow cytometry. Results DihydrotanshinoneⅠ effectively inhibited the proliferation and migration of EC9706 cells. DihydrotanshinoneⅠ down-regulated β-catenin, cyclin D1 and c-myc protein expression in cells (P<0.05). DihydrotanshinoneⅠ promoted apoptosis in a dose-dependent manner. Conclusions DihydrotanshinoneⅠ can inhibit proliferation and migration of EC9706 cells and promote the cells apoptosis.

Key words: dihydrotanshinoneⅠ, esophageal squamous cell carcinoma cell line EC9706, Wnt/β-catenin signaling pathway

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