Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (11): 1500-1505.

• Original Articles • Previous Articles     Next Articles

Inhibition of miR-621 expression promotes proliferation, migration and invasion of human gastric cancer cell lines

CHEN Guo-yan, FENG Xiao-bo*   

  1. Department of Gastroenterology, the Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China
  • Received:2019-08-26 Revised:2020-02-08 Online:2020-11-05 Published:2020-10-30
  • Contact: *

Abstract: Objective To detect the expression of miR-621 in gastric cancer tissues and cell lines and to evaluate the prognostic significance of miR-621, and to analyze the effect of miR-621 on the biological function of gastric cancer cells. Methods Expression of miR-621 in cancer tissue and cell lines was detected by real-time polymorperase chain reaction(RT-qPCR). Chi-square test was used to analyze the relationship between miR-621 and clinical pathological features of gastric cancer patients. Prognostic significance of miR-621 for gastric cancer was analyzed by survival curve and Cox regression. Finally, human gastric cancer cells were transfected with miR-621 mimics and inhibitors to analyze the proliferation, migration and invasion ability of gastric cancer cells. Results The expression of miR-621 was significantly downregulated in gastric cancer tissues and cell lines as compared to the adjacent tissues and gastric mucosa cell lines(P<0.01), and low expression was associated with lymph node metastasis, tumor differentiation, and TNM stage in gastric cancer(P<0.05). The patients with low expression of miR-621 had a poor overall survival rates(log-rank test P<0.001), and miR-621 was an independent prognostic indicator for gastric cancer patients(HR=3.09, 95% CI=1.31-7.27, P=0.01). In addition, low-expression of miR-621 significantly increased proliferation, migration, and invasion of gastric cancer cells(P<0.05). Conclusions Low expression of miR-621 is associated with poor prognosis of gastric cancer, which promotes the proliferation, migration and invasion of gastric cancer cells.

Key words: gastric cancer, miR-621, prognosis, biological function

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