Abstract: The next-generation sequencing technology (NGS) is one of the most powerful tools in modern science research with high sensitivity and specificity. Connective tissue disease is a kind of rheumatic diseases characterized by connective tissue involvement. This paper reviews the principles and the characteristics of the NGS，research profiles of connective tissue disease and its frequent diseases with a particular focus on the application in the connective tissue disease．In the end, the existing problems of NGS in life science research and future directions are prospected．

Key words: Connective tissue diseases, rheumatic diseases, Next-generation sequencing technology