Abstract: Objective To identify the role of miR-24 in human colorectal cancer SW620 cell proliferation. Methods SW620 cells were cultured in vitro. The miR-24 was predicted with bioinformatics and identified with dual luciferase report system. Expressions of miR-24 and CARMA3 were determined with Real-time PCR and Western blot after transfection of miR-24. The proliferation of SW620 cells was detected in vitro by CCK8. Results miR-24 can inhibit CARMA3 expression by binding to 3’ UTR of CARMA3 mRNA. miR-24 can negative control the CARMA3 expression. The proliferation of SW620 cells was negative controlled by miR-24. Conclusion The miR-24 may negatively regulate CARMA3 expression by binding to its 3’ UTR in human colorectal cancer SW620 cells and cell proliferation.

Key words: MiRNA, Colorectal neoplasm, CARMA3, Cancer