Abstract: The blood-brain barrier (BBB) is a significant physiological barrier segregated between the central neural system and peripheral blood circulation. It plays an essential role in maintaining the brain homeostasis. Many neurological conditions are accompanied with blood-brain barrier disruption（e.g. traumatic brain injury、intracranial tumor、chronic neurodegenerative disorders）.Recently many experiments have suggested that the blood-brain barrier disruption may be a cause rather than just a result from brain damage which suggested that the BBB is an important therapeutic target. However, the current lack of concise and accurate blood-brain barrier assessment has become a limiting factor in the clinical practice and research of central neuropathy.

Key words: blood-brain barrier, neural disease , experimental methodology