Abstract: Objective To investigate the effect of β1-Adrenoceptor autoantibody on liver function. Methods The biologically active of β1-AA was prepared and passive immunization model was established with β1-AA. The biochemical parameters of the liver were measured by the automatic serum biochemical analyzer. The liver size, hepatic vein, portal vein velocity were detected by liver ultrasound; hepatocytes apoptosis were tested by tunel staining, Annexin V / PI staining and caspase 3 activity detection. Results The biologically active of β1-AA and passive immunization model were established successfully. The ALT and AST of the liver significantly increased and the ALB decreased in the passive immunization process. The apoptosis of the hepatocytes increased, and metoprolol could partially reversed this effect. Conclusion β1-AA can induce hepatocytes apoptosis by β1-adrenergic receptor and participate in the development of liver injury.

Key words: β1-adrenergic receptor autoantibody hepatic cells apoptosis