Abstract: Objective To investigate whether curcumin could inhibit cisplatin-induced apoptosis of HK-2 cells. Methods Using MTT methods to observe inhibitory effects of curcumin and cisplatin on the proliferation of HK-2 cell. Detecting caspases expression after curcumin and cisplatin treatment by Western blot. Results To some extent, cisplatin can induce cell apoptosis, and its effect is dose-dependent(P<0.05).Under the joint effect of curcumin and cisplatin on HK-2 cell，the number of apoptosis decreased(P<0.05), yet in the same time the expressions of Bcl-2 protein were increased(P<0.05) and the expressions of Bcl-2 protein were decreased(P<0.05). Conclusion Cisplatin enhances the apoptosis of HK-2 cell in a dose-dependent relationship within limits, and possess the antagonistic effect when cisplatin and curcumin are conbined. Curcumin can antagonize cisplatin-induced apoptosis in HK-2 by regulating the expressions of Bax and Bcl-2 protein.

Key words: curcumin , cisplatin, HK-2 cell, apoptosis