Abstract: Objective To investigate the effect of obestatin on the apoptosis of rat pancreatic islet cell line INS1 induced by high glucose.Methods INS1 cells were cultured in different concentration of glucose.The survival rate and proliferation of INS1cells detected by MTT method;Hoechst 33258 nuclear staining was used to detect nuclear morphology. Caspase-3 method was used to study the relationship between the protective effect of obestatin and the PI3K pathway; Finally，using real time PCR detection of FOXO1and SREBP1c, Bax, PDX-1expression, to further clarify the protective effect of obestatin on cells. Results Under high glucose condition,obestatin promoted the proliferation of INS1 cells at 100nmol/L,and promoted the proliferation of INS1 cells significantly(P<0.01 compared with the control group and high glucose group).Obestatin can reduce high glucose-induced apoptosis(P<0.01).The expressions of FOXO1,SREBP1c,Bax and PDX-1 were decreased,while the expressions of FOXO1,SREBP1c,Bax and PDX-1 were increased in the high glucose group; Conclusions OB can attenuate the injury of INS1 cells induced by high glucose in rats.

Key words: Obestatin, Glucotoxicity, β cells, protection