Abstract: Objective To observe the expression of relative protein in the tissue of colon rectum cancer(CRC) patients with diabets(Db) in EMT progress. Methods Collecting some tissues, including the tumor tissues of CRC patients combining with diabets(Db)and nontumorous tissues. All the tissues were divided into control group and tumor group. The tumor group was divided into group with Db and without Db, according to the existance of Db. Associated protein RAGE, AGE, E-cad, β-catenin and vimentin were detected by immunohistochemical staining. The expression levels of these protein were measured by Western blot. Results RAGE, AGE, E-cad and vimentin were mainly located in cell membrane, β-catenin was mainly located in cytoplasm, and the two tumor groups were stained deeper than control group(P<0.01).The expression levels of AGE, RAGE, β-catenin and vimentin in two tumor groups were higher than those in control group, E-cad was lower than it(P<0.01). Compaerd with CRC without Db group, the expression levels of AGE, RAGE, β-catenin and vimentin in the CRC with Db group were up-regulated, E-cad was lower than it in the same differential degree(P<0.01). Conclusions the relative protein β-catenin and vimentin are up-regulated, E-cad is down-regulated in the tissue of CRC patients with Db. The EMT progress is promoted and it has played an play a vital role in enhancement to the development of CRC.

Key words: Colon rectum cancer(CRC), Tumor, Epithelial-Mesenchymal Transitions(EMT), Diabet