Basic & Clinical Medicine ›› 2016, Vol. 36 ›› Issue (5): 688-693.
Objective To identify human GIPC2 core promoter and potential cis-acting element using sequence homology. Methods Homologous sequence of GIPC2 was identified by comparing GIPC2 coding and non-coding sequence from different species in Vista database. A series of luciferase constructs comprising human or rat GIPC2 sequences truncated at various lengths upstream of translation initiation site were tested in HEK293T and PC12 to confirm the core promoter sequence. Conserved intronic GIPC2 sequences were each inserted upstream of human core promoter to test for potential cis-acting regulatory activities. Results The human GIPC2 core promoter sequence spans from +32 to +190 upstream of translation initiation site. A homologous sequence 1500bp upstream of human GIPC2 translation initiation sites represses GIPC2 promoter activity and a homologous sequence from the first intron promotes GIPC2 promoter activity. Conclusions we have identified human GIPC2 core promoter and potential cis-acting element using sequence homology.
Key word: GIPC2,
homologous sequence ,
gene expression regulation
cheng zhikai. Identification of human GIPC2 core promoter and potential cis-acting element using sequence homology approach [J]. Basic & Clinical Medicine, 2016, 36(5): 688-693.
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