Abstract: Objective To investigate the effect of lentivirus-mediated shRNA silencing angiopoietin-like protein 4(ANGPTL4) gene on migration and invasion of cervical cancer cell line SiHa. Methods In this experiment, three groups were designed: control, negative control and LV3/ANGPTL4 groups. The shRNA for lentiviral interference vectors were infected into SiHa cells. The expressions of Integrinβ1, VEGF and MMP9 protein were detected by Western blot. The migration and invasion ability of SiHa cells were detected by scratch wound assay and Transwell respectively. Results ANGPTL4 shRNA lentiviral interference vectors were successfully infected into SiHa cells. Compared with those in control group and negative control group, the expressions of Integrinβ1, VEGF and MMP9 protein in LV3/ANGPTL4 group were down-regulated(P<0.01). The migration and invasion ability of SiHa cells in LV3/ANGPTL4 group were significantly decreased (P<0.05). Conclusion Lentiviral-shRNA vector targeting ANGPTL4 gene can down-regulate the expression of Integrinβ1, VEGF and MMP9 protein in SiHa cells. It also can effectively inhibit the migration and invasion ability of Siha cells.

Key words: angiopoietin-like protein 4, lentivirus, migration, invasion