Abstract: Objective To identify novel lncRNAs involved in cerebellar neurogenesis and validate the expression of related splice variants. Methods Microarray analysis of infant (P7) and adult (P28) mice cerebellar RNA was performed to discover novel lncRNAs involved in mice cerebellum neurogenesis. RT-qPCR was then performed to validate the expression of candidate lncRNAs and splice variants. Results A novel lncRNA, Gm2694, was found to be specifically expressed in mice cerebellum in a developmentally regulated manner. In total 8 kinds of splice variants has been detected in cerebellum with various expression levels: besides 7 variants as predicted in the Ensembl databases, another novel variant was identified in mice cerebellum by RACE cDNA amplification. Conclusion Temporal spatial analysis reveals that Gm2694 is a novel lncRNA possibly involved in mice cerebellum neurogenesis.

Key words: long non-coding RNA, Gm2694, mice cerebellar neurogenesis, splice variant