Abstract: Objective To investigate whether H2S can inhibit resistin secretion in mouse insulin-resistant 3T3-L1 adipocytes via activation of amp-activated protein kinase. Methods The cells were divided into the normal groups, the insulin resistance groups and the 50 umol/L NaHS treatmen tgroups. The ELISA method was used to analyze the level of resistin secretion, Western blot was used to analyze the expression of activated protein kinase (AMPK) and acetyl coenzyme A carboxylase enzyme (ACC) phosphorylation protein and RT-PCR was performed to analyzed the expression of resistin mRNA and AMPK mRNA. Results The effects of NaHS significantly decreased the secretion level of resistin compared with the control group（P<0.05）. The expression of AMPK and ACC protein phosphorylation in normal and insulin resistant cells significantly increased（P<0.05）. The AMPK pathway inhibitor compound C markly weakened the effects on AMPK and ACC protein phosphorylation. At the same time, the secretion level of resistin was inhibited. Conclusion Hydrogen sulflide may inhibits resistin secretion in mouse insulin-resistant 3T3-L1 adipocytes via activation of AMP-activated protein kinase.

Key words: hydrogen sulfide, amp-activated protein kinase, 3T3-L1 adipocytes, resistin