Basic & Clinical Medicine ›› 2015, Vol. 35 ›› Issue (7): 867-872.
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Abstract: Objective To investigate the effects of photodynamic therapy(PDT) on cell cycle and apoptosis in endometrial cancer Ishikawa cell line. Methods Ishikawa cell were treated by PDT with photosensitizer 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin(TMPyP). Cell morphology were observed by inverted microscope. Survival rate of Ishikawa cell was evaluated with Cell Counting Kit-8 (CCK-8). Cell cycle was analyzed by flow cytometry (FCM). Cell apoptosis was analyzed with Annexin V-FITC/PI Apoptosis Kit for FCM. The expression of Bcl-2、NF-κB P65 and phospho-NF-κB P65(p-NF-κB P65) and were detected by Western blotting. Results PDT could cause Ishikawa cell morphological changes. Survival rate decreased in Ishikawa cell treated by PDT after 24h(P<0.05) and the rate was related with photosensitizer concentration and energy intensity. FCM analysis indicated PDT could induce S phase arrest and apoptosis(P<0.05). The expression of Bcl-2 protein and phosphorylation of NF-κB P65 were down regulated in Ishikawa cell after PDT(P<0.05). Conclusion PDT could induce S phase arrest and apoptosis in endometrial cancer Ishikawa cell , the possible mechanism is down-regulation of Bcl-2 and NF-κB.
Key words: Key words: endometrial cancer Ishikawa cell, photodynamic therapy, cell cycle, cell apoptosis
CLC Number:
R737.33
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2015/V35/I7/867