Abstract: Objective To assessed the effect of the two Single nucleotide polymorphisms （rs9275572 and rs9275319）in human leukocyte antigen (HLA)-DQ gene on the outcomes of HBV infection in Chinese Han population. Methods HLA-DQ (rs9275572 and rs9275319) were genotyped in 921 subjects by using the TaqMan probe method, including 310 HBV-related chronic liver disease patients (CLD), 295 HBV spontaneous clearance subjects (SC) and 316 healthy control individuals (HC). Results 1) Rs9275572 AG genotype showed a significant protective effect on HBV clearance (OR，1.82，95%CI, 1.26~2.62; dominant genetic model: OR, 1.84, 95% CI, 1.30~2.61). 2) SNP rs9275319 showed a significant association with HBV susceptibility and HBV natural clearance. The rs9275319 C allele play a protective role (the allele model OR, 0.494, 95% CI, 0.332~0.735; the dominant genetic model OR, 0.47, 95%CI,0.31~0.72 for CLD vs HC; the allele model, OR, 1.610, 95%CI, 1.065~2.434; the dominant genetic model OR, 1.57; 95%CI, 1.01~2.48 for CLD vs SC). 3) a conspicuous association was unveiled between haplotype T-G/T-A and HBV susceptibility or spontaneous clearance. Conclusion HLA-DQ gene polymorphisms were correlated with HBV susceptibility or nature clearance.

Key words: Hepatitis B, Single nucleotide polymorphism (SNP), HLA-DQ gene