Abstract: Objective To explore the interaction and mechanism between miR-26b and lncRNA SNHG5. Methods Quantitative real-time PCR analyzed the differential expression of SNHG5 in normal gastric epithelial cell GES-1 compared with it in gastric cancer cell. Transient transfected MGC-803 cells with SNHG5 overexpressed plasmid and detect the expression level of miR-26b and pri-miR-26b by Taq Man PCR analysis. Meanwhile, transient transfected MGC-803 cells with miR-26b mimics to detect the expression change of SNHG5. Results Expression level of SNHG5 was significantly higher in gastric cancer cells than in normal gastric epithelial cell GES-1 (P<0.05). SNHG5 inhibited the expression of miR-26b and pri-miR-26b(P<0.01), but miR-26b could not influence the expression of SNHG5. Conclusion SNHG5 inhibited the expression of miR-26b, but the mechanism need to be further clarified.

Key words: Gastric cancer, SNHG5, miR-26b