Abstract: Objective To investigate whether the high glucose Dulbecco’s Modified Eagle Medium and fetal bovine serum as base fluid are benefit for improve the viability and post-transplant survival rate of frozen adipose tissue. Methods Fat granules was cryopreserved at -180℃ in physiological saline, high glucose Dulbecco’s Modified Eagle Medium and fetal bovine serum respectively, adding with 8%DMSO. Rewarming at 4 weeks, the viability and post transplantation survival rate of frozen fat were determined by trypan blue staining, creatine kinase activity assay, MTT assay and xenotransplantation experiments. Results Frozen adipose tissue in fetal bovine serum adding with 8%DMSO showed an increase in cell viability and improvement in post transplantation survival rate (p<0.01). Conclusion FBS as a base fluid can significantly improve the cell vitality and post transplantation survival rate of cryopreservation adipose tissue.

Key words: adipose tissue, cryopreservation, viability, post-transplant survival rate